Single-Stranded DNA Binding Protein

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits that cooperatively bind to single-stranded DNA with high affinity. This protein is involved in DNA replication and in recombination in vivo.

Applications

• Studying DNA replication and recombination.
• DNA sequencing using the primer walking strategy with contiguous oligonucleotides.

References

1. Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
2. Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
3. Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
4. Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.

Storage Buffer: 50mM Tris-HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% (v/v) glycerol.

Source: E. coli.

QC Tests: Protein concentration, DNase, RNase, SDS-PAGE/purity, endonuclease.