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Am. J. Hum. Genet. 66, 790-818. Minor Lesion Mutational Spectrum of the Entire NF1 Gene Does Not Explain Its High Mutability but Points to a Functional Domain Upstream of the GAP-Related Domain. 2000

Fahsold, R. , Hoffmeyer, S. , Mischung, C. , Gille, C. , Ehlers, C. , Kucukceylan, N. , Abdel-Nour, M. , Gewies, A. , Peters, H. , Kaufmann, D. , Buske, A. , Tinschert, S. , Nurnberg, P.

Notes: Reverse transcription reaction was performed in the presence of 25ug/ul Single-Stranded DNA Binding Protein and 1 unit/ul RNasin® Ribonuclease Inhibitor. PCR product and 35S-methionine were added to the TNT® Coupled Reticulocyte Lysate System to produce labeled protein in a PTT assay. (1185)

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