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S1 Nuclease

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Endonuclease Specific for Single-Stranded DNA and RNA

  • Enzyme yields 5´-phosphoryl-terminated products
  • Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) resist degradation except with extremely high concentrations of enzyme
  • Supplied at a concentration of 20–100u/μl


Catalog number selected: M5761

$ 45.00
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S1 Nuclease
$ 45.00
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S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. S1 Nuclease is provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO4.


  1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
  2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
  3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.

S1 Nuclease


What's in the box?

Item Part # Size

S1 Nuclease

E576B 1 × 10,000u

S1 Nuclease 10X Reaction Buffer

M577A 1 × 1ml


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Download SDSPDF (216 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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