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S1 Nuclease

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Endonuclease Specific for Single-Stranded DNA and RNA

  • Enzyme yields 5´-phosphoryl-terminated products
  • Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) resist degradation except with extremely high concentrations of enzyme
  • Supplied at a concentration of 20–100u/μl


Catalog number selected: M5761

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S1 Nuclease
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S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. S1 Nuclease is provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO4.


  1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
  2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
  3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.

Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl2, 50mM NaCl and 50% (v/v) glycerol.

Source: Fungal α amylase powder.

QC Tests: Activity, unidirectional deletions using the Erase-a-Base™ System.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.


What's in the box?

Item Part # Size

S1 Nuclease

E576B 1 × 10,000u

S1 Nuclease 10X Reaction Buffer

M577A 1 × 1ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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