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Genome Res. 21, 1738-45. Application of microdroplet PCR for large-scale targeted bisulfite sequencing 2011

Komori, H.K., LaMere, S.A., Torkamani, A., Hart, G.T., Kotsopoulos, S., Warner, J., Samuels, M.L., Olson, J., Head, S.R., Ordoukhanian, P., Lee, P.L., Link, D.R. and Salomon, D.R.

Notes: The authors of this study sought to correlate promoter methylation with gene expression. Gene expression data was generated by RNA-seq of Jurkat cells. Amplified cDNA was prepared from total RNA, the cDNA was treated with S1 nuclease to remove single stranded nucleic acids and used as template for the sequencing libraries. (4536)

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S1 Nuclease

J. Biol. Chem. 273, 23454-23462. Identification and characterization of a novel tissue-specific transcriptional activating element in the 5'-flanking region of the CYP2A3 gene predominantly expressed in rat olfactory mucosa 1998

Zhang, J., Ding, X.

Notes: The Core Footprinting System was used to footprint a region of the CYP2A3 gene with nuclear extracts derived from various rat and mouse tissues. The RNasin® Ribonuclease Inhibitor was used to protect transcripts of a eukaryotic in vitro transcription reaction. The transcripts were used in an S1 Nuclease protection assay. (0091)

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