Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, endonuclease, 3´ overhang protection assay.
Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble nucleotides from double-stranded DNA in 30 minutes at 37°C.
- Generating nested sets of deletions with double-stranded, linear DNA.
- Preparation of single-stranded DNA.