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Ribonuclease H

M4281_RNase-H--50u_3
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Endonuclease that Specifically Hydrolyzes the Phosphodiester Bonds of RNA Hybridized to DNA

  • Removes the RNA strand prior to second-strand cDNA synthesis
  • Analyzes in vitro polyadenylation reaction products
  • Supplied at a concentration of 0.5–2u/μl

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Catalog number selected: M4281

$ 207.00
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Ribonuclease H
50u
$ 207.00
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Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.

Reference

  1. Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.

Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.

Source: Recombinant E. coli strain.

QC Tests: Activity, DNase, RNase, endonuclease, cDNA synthesis.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).

Specifications

You are viewing: M4281 Change Configuration

What's in the box?

Item Part # Size

RNase H

M428A 1 × 50u

SDS

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Download SDSPDF (41 KB) – Deutsch (Deutschland)

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

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Specifications

You are viewing: M4285 Change Configuration

What's in the box?

Item Part # Size

RNase H

M428C 1 × 250u

SDS

Choose language:

Download SDSPDF (41 KB) – Deutsch (Deutschland)

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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