We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Have you purchased from Promega in the last six months? Take our brief survey ›

AMP-Glo™ Assay

Monitor AMP Concentration in Biochemical Reactions

  • Use with virtually with any AMP-producing enzyme
  • Well-suited for low-activity enzymes
  • Sensitive to low AMP concentration

Size

Catalog number selected: V5011

$ 710.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
AMP-Glo™ Assay
1,000 assays
$ 710.00
Your price: Log in
Change Configuration

Sensitive, Luminescent AMP Detection 

The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP.  It can be used to measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. 

The assay quantitatively monitors AMP concentration in a biochemical reaction in a wide range of plates, including high-throughput formats. The stable luminescent signal eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate.

Two reagents are supplied: one to terminate the AMP-generating enzymatic reaction, remove ATP and convert AMP produced into ADP, and a second reagent to convert ADP to ATP, which is used to generate a luminescence in a luciferase reaction. The AMP-Glo™ Assay is well suited for monitoring AMP produced in biochemical reactions catalyzed by enzymes that do not use ATP as a substrate, such as cAMP-dependent phosphodiesterases (PDE) and bacterial DNA ligases.


    AMP-Glo™ Assay principle.

    Screen Library Compounds for Effects on AMP-Producing Enzymes 

    The AMP-Glo™ Assay has a high dynamic range and produces a strong signal at low substrate conversion, allowing screening of low-activity enzymes. The assay produces minimal false hits and Z´ values greater than 0.7, and can be used to screen library compounds for effects on target enzymes such as:

    • ubiquitin ligases
    • cyclic AMP-specific phosphodiesterases
    • aminoacyl-tRNA synthetases
    • DNA ligases
    • poly(A) deadenylases
    • demethylases (indirect method)

    AMP-Glo™ Assays contain enough reagent to perform the indicated number of reactions in a low-volume 384-well plate format using 5μl of reaction enzyme, 5μl of AMP-Glo™ I Reagent and 10μl of AMP Detection Solution. The system also can be used in standard 96-well plates (25μl:25μl:50μl).

    Specifications

    You are viewing: V5011 Change Configuration

    What's in the box?

    Item Part # Size
    AMP-Glo™ Reagent I V503A 1 × 5ml
    AMP-Glo™ Reagent II V504A 1 × 100μl
    Kinase-Glo® One Solution V505A 1 × 10ml
    AMP, 10mM V506A 1 × 100μl

    Ultra Pure ATP, 10mM

    V915A 1 × 500μl

    SDS

    Choose language:

    Download SDS PDF (0 B)

    Certificate of Analysis

    See all certificates for V5011

    Search

    View more results

    No results

    Loading…

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

    Storage Conditions

    BB

    Specifications

    You are viewing: V5012 Change Configuration

    What's in the box?

    Item Part # Size
    AMP-Glo™ Reagent I V503B 1 × 50ml
    AMP-Glo™ Reagent II V504B 1 × 1ml
    Kinase-Glo® One Solution V505B 1 × 100ml
    AMP, 10mM V506B 1 × 1ml

    Ultra Pure ATP, 10mM

    V915B 1 × 5ml

    SDS

    Choose language:

    Download SDS PDF (0 B)

    Certificate of Analysis

    See all certificates for V5012

    Search

    View more results

    No results

    Loading…

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

    Storage Conditions

    BB

    Let's find the product that meets your needs.

    Talk to a Scientist

    Sabine

    Sabine

    Germany