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AMP-Glo™ Assay

V5011_AMP-Glo--Assay--1000-Assays_3
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Monitor AMP Concentration in Biochemical Reactions

  • Use with virtually with any AMP-producing enzyme
  • Well-suited for low-activity enzymes
  • Sensitive to low AMP concentration

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Catalog number selected: V5011

$ 715.00
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AMP-Glo™ Assay
1,000 assays
$ 715.00
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Sensitive, Luminescent AMP Detection 

The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP.  It can be used to measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. 

The assay quantitatively monitors AMP concentration in a biochemical reaction in a wide range of plates, including high-throughput formats. The stable luminescent signal eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate.

Two reagents are supplied: one to terminate the AMP-generating enzymatic reaction, remove ATP and convert AMP produced into ADP, and a second reagent to convert ADP to ATP, which is used to generate a luminescence in a luciferase reaction. The AMP-Glo™ Assay is well suited for monitoring AMP produced in biochemical reactions catalyzed by enzymes that do not use ATP as a substrate, such as cAMP-dependent phosphodiesterases (PDE) and bacterial DNA ligases.

AMP-Glo™ Assay principle.

Screen Library Compounds for Effects on AMP-Producing Enzymes 

The AMP-Glo™ Assay has a high dynamic range and produces a strong signal at low substrate conversion, allowing screening of low-activity enzymes. The assay produces minimal false hits and Z´ values greater than 0.7, and can be used to screen library compounds for effects on target enzymes such as:

  • ubiquitin ligases
  • cyclic AMP-specific phosphodiesterases
  • aminoacyl-tRNA synthetases
  • DNA ligases
  • poly(A) deadenylases
  • demethylases (indirect method)

AMP-Glo™ Assays contain enough reagent to perform the indicated number of reactions in a low-volume 384-well plate format using 5μl of reaction enzyme, 5μl of AMP-Glo™ I Reagent and 10μl of AMP Detection Solution. The system also can be used in standard 96-well plates (25μl:25μl:50μl).

See More Data: This peer-reviewed paper demonstrates how the AMP-Glo™ Assay was used to monitor and characterize soluble and membrane-bound ectonucleotidases CD73 and CD39.

Read the Paper
60109223-ampglo-paper-image-1030x1450

Specifications

You are viewing: V5011 Change Configuration

What's in the box?

Item Part # Size
AMP-Glo™ Reagent I V503A 1 × 5ml
AMP-Glo™ Reagent II V504A 1 × 100μl
Kinase-Glo® One Solution V505A 1 × 10ml
AMP, 10mM V506A 1 × 100μl

Ultra Pure ATP, 10mM

V915A 1 × 500μl

SDS

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Download SDSPDF (139 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5012 Change Configuration

What's in the box?

Item Part # Size
AMP-Glo™ Reagent I V503B 1 × 50ml
AMP-Glo™ Reagent II V504B 1 × 1ml
Kinase-Glo® One Solution V505B 1 × 100ml
AMP, 10mM V506B 1 × 1ml

Ultra Pure ATP, 10mM

V915B 1 × 5ml

SDS

Choose language:

Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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