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ADP-Glo™ Max Assay

Luminescent Method to Measure ATPase or Kinase Activity

  • Screen library compounds for effects on target enzymes
  • Perform mode of action studies on enzymes of interest
  • Measure activity of immunoprecipitated ATPases/kinases
  • Use when higher ATP concentration is required (up to 5mM)

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Catalog number selected: V7001

$ 649.00
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ADP-Glo™ Max Assay
1,000 assays
$ 649.00
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The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction. The assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) when higher ATP concentration is required (up to 5mM). The ADP-Glo™ Max Assay produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats.

The assay is performed in two steps: first, after the completion of the ADP-producing reaction, an equal volume of ADP-Glo™ Reagent is added to terminate the reaction and deplete the remaining ATP. Second, the ADP-Glo™ Max Detection Reagent is added to simultaneously convert ADP to ATP, and the latter is converted to light in a coupled reaction with luciferase/luciferin.

The ADP-Glo™ Max Assay has a high dynamic range and produces a strong signal at low ATP to ADP conversion, making it well suited for screening low-activity ATPases such as drug membrane transporters and heat shock proteins. The assay produces minimal false hits and Z´ values of greater than 0.7.

We offer a large selection of Kinase Enzyme Systems. Visit www.promega.com/kinase/ to see the collection.

  • High signal strength at low ATP conversion makes the assay well suited for low-activity ATPases/kinases.
  • Sensitive to low concentrations of ADP, requiring less enzyme than other assays.
  • Use with virtually with any ADP-producing enzyme—enables researchers to screen a wider range of enzymes using a single platform.
  • Can be used at ATP concentrations up to 5mM, important for enzymes with high Kam values for ATP and for mode of action studies.
  • Accurately measures ADP levels at a wide range of starting ATP concentrations; users assured that activity measured truly reflects enzyme activity and produces accurate IC50 values comparable to radioactivity-based assays.

The ADP-Glo™ Max Assays contain enough reagent to perform the indicated number of reactions in 384-well format using 5μl, 5μl and 10μl of an enzyme reaction, ADP-Glo™ Reagent and ADP-Glo™ Max Detection Reagent, respectively, per sample.

Principle of the ADP-Glo™ Max Assay.

Principle of the ADP-Glo™ Max Assay.
Principle of the ADP-Glo™ Max Assay.

Specifications

You are viewing: V7001 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Max Detection Buffer

V701A 1 × 10ml

ADP-Glo™ Max Detection Substrate

V702A 1 × 1 vial

Ultra Pure ATP, 100mM

V703A 1 × 250μl

ADP-Glo™ Reagent

V912A 1 × 5ml

ADP, 10mM

V916A 2 × 500μl

SDS

Choose language:

Download SDSPDF (140 KB) – English (United States)

Certificate of Analysis

See all certificates for V7001

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Specifications

You are viewing: V7002 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Max Detection Buffer

V701B 1 × 100ml

ADP-Glo™ Max Detection Substrate

V702B 1 × 1 vial

Ultra Pure ATP, 100mM

V703B 1 × 2.5ml

ADP-Glo™ Reagent

V912B 1 × 50ml

ADP, 10mM

V916B 1 × 5ml

SDS

Choose language:

Download SDSPDF (140 KB) – English (United States)

Certificate of Analysis

See all certificates for V7002

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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