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YES1-NanoLuc® Fusion Vector

Kinase Fusion Vector for NanoBRET™ Target Engagement

  • Transfection-ready DNA to express full-length YES1 kinase fused to NanoLuc® luciferase
  • Measures kinase target engagement in live cells
  • Use with NanoBRET™ TE Intracellular Kinase Assay, K-4

Size

Catalog number selected: NV2241

$ 500.00
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This product is discontinued
YES1-NanoLuc® Fusion Vector
20μg
$ 500.00
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36566039-NanoBRET-TE-Kinases-Overview-Image

Express Full-Length NanoLuc®-Kinase Fusions in Mammalian Cells

The YES1-NanoLuc® Fusion Vector contains the coding region of NanoLuc® luciferase fused to the C-terminus of human YES1 kinase. It is designed for use with the NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay, K-4, where the plasmid can be transfected into various cell lines for YES1 target engagement analysis. The NanoLuc® luciferase kinase fusion vectors are supplied transfection-ready. The CMV promoter drives the expression of the full-length NanoLuc® kinase fusion protein. 

Vector Details:

About Kinase Fusion Vector Names

The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET™ TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3' to the kinase gene (at the C-terminus of the kinase).

NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET™ TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET™ tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET™ signal for estimating intracellular affinity against the target protein. The NanoBRET™ tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table on the NanoBRET™ TE Intracellular Kinase Assay page shows which NanoBRET™ tracer assay (K-4 or K-5) is correct for each available kinase fusion vector.

Specifications

What's in the box?

Item Part # SizeConcentration

YES1-NanoLuc® Fusion Vector

NV224A 1 × 20μg1μg/μl

Certificate of Analysis

See all certificates for NV2241

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

Licensed from Kazusa DNA Research Institute.

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