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NanoLuc®-NEK11 Fusion Vector

Kinase Fusion Vector for NanoBRET™ Target Engagement

  • Transfection-ready DNA to express full-length NEK11 kinase fused to NanoLuc® Luciferase
  • Measures kinase target engagement in live cells
  • Use with NanoBRET™ TE Intracellular Kinase Assay, Adherent Format protocol

Size

Catalog number selected: NV3771

$ 510.00
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This product is discontinued
NanoLuc®-NEK11 Fusion Vector
20μg
$ 510.00
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Express Full-Length NanoLuc®-Kinase Fusions in Mammalian Cells

The NanoLuc®-NEK11 Fusion Vector contains the coding region of NanoLuc® luciferase fused to the N-terminus of human NEK11 kinase. It is designed for use with the NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay, Adherent Format protocol, where the plasmid can be transfected into various cell lines for NEK11 target engagement analysis. The NanoLuc® luciferase kinase fusion vectors are supplied transfection-ready. The CMV promoter drives the expression of the full-length NanoLuc® kinase fusion protein. 

Vector Details:

  • Gene name: NEK11
  • Sequence
  • Recommended Assay: NanoBRET™ TE Intracellular Kinase, K-10
  • Example data using Tracer: K-10

About Kinase Fusion Vector Names

The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET™ TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).

NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET™ TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET™ Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET™ signal for estimating intracellular affinity against the target protein. The NanoBRET™ Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET™ Tracer assay is correct for each kinase fusion vector.

Specifications

What's in the box?

Item Part # SizeConcentration

NanoLuc®-NEK11 Fusion Vector

NV377A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (189 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NanoLuc® ORF TE Vector Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

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