About Kinase Fusion Vector Names
The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET™ TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).
NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays
The NanoBRET™ TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET™ Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET™ signal for estimating intracellular affinity against the target protein. The NanoBRET™ Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET™ Tracer assay is correct for each kinase fusion vector.