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CCNB1 Expression Vector

NV2601_CCNB1 Expression Vector- 20ug

Regulatory Vector for NanoBRET™ Target Engagement

  • Transfection-ready DNA to express full-length CCNB1 regulatory protein
  • Use with select kinase-NanoLuc® fusion vectors and NanoBRET™ TE Intracellular Kinase Assay

Size

Catalog number selected: NV2601

$ 510.00
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This product is discontinued
CCNB1 Expression Vector
3 × 20μg
$ 510.00
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Express Regulatory Proteins in Mammalian Cells

The CCNB1 Expression Vector contains the coding region of the human CCNB1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The Expression Vector and kinase-NanoLuc® Fusion Vector are cotransfected into cells prior to analysis using the appropriate NanoBRET™ Target Engagement Intracellular Kinase Assay. These regulatory protein Expression Vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.

For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein Expression Vector, see the NanoBRET™ Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.

Vector Details:

  • Gene name: CCNB1
  • Sequence
  • Recommended Assay: NanoBRET™ TE Intracellular Kinase Assay, Adherent Format

About Kinase Fusion Vector Names

The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET™ TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).

NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET™ TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET™ Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET™ signal for estimating intracellular affinity against the target protein. The NanoBRET™ Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET™ Tracer assay is correct for each kinase fusion vector.

Specifications

What's in the box?

Item Part # SizeConcentration

CCNB1 Expression Vector

NV260A 3 × 20μg1μg/μl

SDS

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Download SDSPDF (188 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Licensed from Kazusa Genome Technologies.

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