WNK1 Kinase Enzyme System

Kinase Enzyme Systems for Detecting Kinase Activity
View information on global supply logistics
customize-this-small

Easily Screen and Profile WNK1 Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: V5084

$ 545.00
Your price:
Add to Cart
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
WNK1 Kinase Enzyme System
10µg
$ 545.00
Your price: Log in
Change Configuration

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The WNK1 Kinase Enzyme System contains:

  • WNK1 Kinase, 10μg (Human, recombinant; amino acids 181–507). MW: ~67kDa.
  • Native Swine Myelin Basic Protein (MBP) Substrate.
  • Reaction Buffer, MnCl2, DTT.

Recombinant human WNK1 (181–507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. WNK1 is a member of the WNK subfamily of serine/threonine protein kinases that is a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in WNK1 have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK1 is a regulator of blood pressure and deficiency of this protein in mice lowers the blood pressure. WNK1 can regulate the Ca2+-sensing and the subsequent Ca2+-dependent interactions mediated by synaptotagmin C2 domains, and WNK1 exhibits additive CFTR inhibition.

WNK1 NCBI Database Entry.

The WNK1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

You are viewing: V5084 Change Configuration

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V6599 Change Configuration

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V5085 Change Configuration

What's in the box?

Item Part # Size

WNK1 Kinase Enzyme System

V5084 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK