The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKACβ Kinase Enzyme System contains:
- PKACβ Kinase, 10μg (Human, recombinant; full length); MW: ~65kDa
- CREBtide Substrate (1mg/ml; KRREILSRRPSYR); derived from human CREB1 isoform A (amino acids 109–121)
- 5X Reaction Buffer; DTT, 0.1M
Recombinant full-length human PKACβ was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. The catalytic subunit C-beta of PKA (PKACβ) is a member of the Ser/Thr protein kinase family (the PKA catalytic subunit consist of three gene products: C-alpha, C-beta, and C-gamma) and has been assigned to human chromosome region 1p36.1. PKACβ is derived from a gene distinct from C-alpha and shows tissue-specific expression. At the amino acid level C-alpha and C-beta showed 93% homology.
NCBI Database Entry
The PKACβ Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.