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PKA Kinase Enzyme System

Easily Screen and Profile PKA Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity


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Catalog number selected: V4246

$ 465.00
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This product is discontinued
PKA Kinase Enzyme System
2,500 units
$ 465.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKA Kinase Enzyme System contains:

  • PKA Kinase, 2,500 units (Bovine, recombinant full-length). MW: ~40kDa.
  • Kemptide (LRRASLG) Substrate.
  • Reaction Buffer, DTT.

Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. Intracellular targets include ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.

PKA NCBI Database Entry.

The PKA Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.


See all Kinase Enzyme Systems available from Promega.


You are viewing: V4246 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately


P117A 1 × 100μl100mM View Product

5X Reaction Buffer A

V307C 1 × 1.5ml

cAMP-Dependent Protein Kinase, Catalytic Subunit

V516A 1 × 2,500u


V560A 1 × 1mg


Choose language:

Download SDSPDF (114 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions



You are viewing: V4247 Change Configuration

What's in the box?

Item Part # Size

PKA Kinase Enzyme System

V4246 1 × 2,500 units

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays


Choose language:

Download SDSPDF (237 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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