The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PASK Kinase Enzyme System contains:
- PASK Kinase, 10μg (Human, recombinant; amino acids 981–end). MW: ~66kDa.
- ZIPtide (KKLNRTLSFAEPG) Substrate.
- Reaction Buffer, DTT.
Recombinant human PASK (981–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress. PASK is involved in sensing environmental changes in light intensity, oxygen concentration and redox potential. Through interaction with IRS-1, PASK has been proposed as a counter-regulatory mechanism in insulin and cytokine signaling. PASK can phosphorylate and inactivate glycogen synthase in vitro. Efficient phosphorylation requires residues 444 to 955 of PASK between the PAS and catalytic kinase domain, and this interaction is inhibited by the PAS domain.
PASK NCBI Database Entry.
The PASK Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.