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MINK1 Kinase Enzyme System

Easily Screen and Profile MINK1 Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V3911

$ 449.00
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This product is discontinued
MINK1 Kinase Enzyme System
10µg
$ 449.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The MINK1 Kinase Enzyme System contains:

  • MINK1 Kinase, 10μg (Human, recombinant; amino acids 1–320). MW: ~61kDa.
  • Native Swine Myelin Basic Protein (MBP) Substrate.
  • Reaction Buffer, DTT.

Recombinant human MINK1 (1–320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes, including cell morphology, cytoskeletal rearrangement and survival. Overexpression of kinase-dead mutants of MINK1 leads to enhanced cell spreading, actin stress fiber formation, adhesion to extracellular matrix and decreased cell motility and invasion. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of p38 MAPK downstream of the Raf/ERK pathway.

MINK1 NCBI Database Entry.

The MINK1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

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SDS

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Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V3912 Change Configuration

SDS

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Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V8001 Change Configuration

What's in the box?

Item Part # Size

MINK1 Kinase Enzyme System

V3911 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

SDS

Choose language:

Download SDSPDF (238 KB) – English (United States)

Certificate of Analysis

See all certificates for V8001

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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