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IGF1R Kinase Enzyme System

V4492

Easily Screen and Profile IGF1R Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V3581

$ 435.00 Your price: Log In

IGF1R Kinase Enzyme System
10µg
$ 435.00
Your price: Log In
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The IGF1R Kinase Enzyme System contains:

  • IGF1R Kinase, 10μg (Human, recombinant; amino acids 960–end). MW: ~53kDa.
  • IGF1Rtide (KKKSPGEYVNIEFG) Substrate; derived from human IRS-1 protein residues 891–902.
  • Reaction Buffer, DTT, MnCl2.

Recombinant human IGF1R (amino acids 960–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. IGF1R (Insulin-like Growth Factor 1) is a transmembrane tyrosine kinase receptor that is activated by IGF-1 and by the related growth factor IGF-2. IGF1R mediates the effects of IGF-1 and plays an important role in growth and anabolic effects in adults. The IGF1R is implicated in several cancers, most notably breast cancer, where it is highly overexpressed and functions as an anti-apoptotic agent by enhancing cell survival. In many instances, the anti-apoptotic properties of IGF1R overexpression allow cancerous cells to resist the cytotoxic properties of chemotherapeutic drugs or radiotherapy.

IGF1R NCBI Database Entry.

The IGF1R Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Item Part # Size

IGF1R Kinase Enzyme System

V3581 1 x 10μg

ADP-Glo™ Kinase Assay

V9101 1 x 1,000 assays

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Patents and Disclaimers

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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