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DNA-Dependent Protein Kinase

Purified DNA-PK for DNA-Binding Protein Phosphorylation Studies

  • Phosphorylates several DNA-binding proteins in vitro, including p53, SV40 large T antigen and several transcription factors.

Size

Catalog number selected: V5811

$ 259.00 Your price: Log In

DNA-Dependent Protein Kinase
2,500u
$ 259.00
Your price: Log In

DNA-Dependent Protein Kinase

DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors. DNA-PK is thought to play a role in controlling gene regulation and cell growth.

DNA-PK is isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa.

Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol.

Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C.

Protocols

Specifications

What's in the box?

Item Part # Size

DNA-Dependent Protein Kinase

V581A 1 × 2,500u

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store at –70°C.

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