Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

DNA-Dependent Protein Kinase

V5811_DNA-Dependent Protein Kinase

Purified DNA-PK for DNA-Binding Protein Phosphorylation Studies

  • Phosphorylates several DNA-binding proteins in vitro, including p53, SV40 large T antigen and several transcription factors.

Size

Catalog number selected: V5811

$ 285.00
Your price:
Please Enquire
This product is discontinued
DNA-Dependent Protein Kinase
2,500u
$ 285.00
Your price: Log in

DNA-Dependent Protein Kinase

DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors. DNA-PK is thought to play a role in controlling gene regulation and cell growth.

DNA-PK is isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa.

Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol.

Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C.

Specifications

What's in the box?

Item Part # Size

DNA-Dependent Protein Kinase

V581A 1 × 2,500u

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store at –70°C.

Resources

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK