We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

DNA-PK Kinase Enzyme System

V4492

Easily Screen and Profile DNA-PK Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: V4106

$ 435.00 Your price: Log In

DNA-PK Kinase Enzyme System
2,500 units
$ 435.00
Your price: Log In
Change Configuration
Kinase-background-v2light

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The DNA-PK Kinase Enzyme System contains:

  • DNA-PK Kinase, 2,500 units (Human, native full-length). MW: ~460kDa (catalytic subunit); ~85kDa (Ku subunit 1); ~70kDa (Ku subunit 2). One unit is the amount of enzyme required to incorporate 1pmol of phosphate into DNA-PK Peptide Substrate (Cat.# V5671) in 1 minute at 30°C.
  • Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol.
  • DNA-Dependent Protein Kinase Peptide Substrate (EPPLSQEAFADLWKK).
  • Reaction Buffer, DNA-PK Activation Buffer (100µg/ml calf thymus DNA in 1X TE.), DTT.

Native human DNA-Dependent Protein Kinase (DNA-PK) purified from HeLa cells. DNA-PK consists of an approximately 460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and an 70kDa peptide. The gene sequence can be found at accession number NM_006904.

DNA-PK NCBI Database Entry.

The DNA-PK Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Protocols

Specifications

You are viewing: V4106 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117A 1 x 100μl100mM View Product

5X Reaction Buffer A

V307C 1 x 1.5ml

DNA-Dependent Protein Kinase Substrate

V567A 1 x 1mg

DNA-Dependent Protein Kinase

V581A 1 x 2,500u

DNA-PK Activation Buffer

V791A 2 x 300μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V4107 Change Configuration

What's in the box?

Item Part # Size

DNA-PK Kinase Enzyme System

V4106 1 x 2,500 units

ADP-Glo™ Kinase Assay

V9101 1 x 1,000 assays

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Resources

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom