We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Microplate Readers are now 20% off! Click to learn more about how you can save!

CK2α2 Kinase Enzyme System

4-tubes

Easily Screen and Profile CK2α2 Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: VA7597

$ 449.00
Your price:
Add to Cart
This product is discontinued
CK2α2 Kinase Enzyme System
10µg
$ 449.00
Your price: Log in
Change Configuration

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CK2α2 Kinase Enzyme System contains:

  • CK2α2 Kinase, 10μg (Human, recombinant, full-length); MW: ~64kDa
  • CK2 Substrate (1mg/ml, RRRADDSDDDDD)
  • 5X Reaction Buffer; DTT, 0.1M

Full-length recombinant human CK2 alpha 2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2α2 or casein kinase II alpha 2 is a member of the CK2 family of Ser/Thr protein kinases. CK2α2 plays a fundamental role in cell function and is involved in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. CK2α2 prefers utilization of acidic proteins such as caseins as substrates. The CK2a2 holoenzyme is a tetramer composed of an alpha chain, an alpha' and two beta chains. The alpha and alpha' chains contain the catalytic site.

Application Note 

NCBI Database Entry

The CK2α2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Specifications

You are viewing: VA7597 Change Configuration

SDS

Choose language:

Download SDSPDF (436 KB) – 日本語 (日本)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: VA7598 Change Configuration

SDS

Choose language:

Download SDSPDF (436 KB) – 日本語 (日本)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: VA7599 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

CK2α2 Kinase Enzyme System

VA7597 1 × 10μg

SDS

Choose language:

Download SDSPDF (594 KB) – 日本語 (日本)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Let's find the product that meets your needs.

Talk to a Scientist

Elisa

Elisa

Spain