We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

CK2α1 Kinase Enzyme System

Easily Screen and Profile CK2α1 Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: V4482

$ 449.00
Your price:
Add to Cart
This product is discontinued
CK2α1 Kinase Enzyme System
10µg
$ 449.00
Your price: Log in
Change Configuration
Kinase-background-v2light

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CK2α1 Kinase Enzyme System contains:

  • CK2α1 Kinase, 10μg (Human, recombinant full-length). MW: ~70kDa.
  • Native Casein Protein Substrate purified from bovine milk.
  • Reaction Buffer, DTT.

Recombinant full-length human CK2α1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2α1 is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth. It is highly expressed in a lympho-proliferative disease of cattle and in many human cancers. Overexpression of the CK2 catalytic subunit in lymphocytes of transgenic mice leads to T cell lymphoma. The highest CK2α1 activity is found in mouse testicles and brain, followed by spleen, liver, lung, kidney and heart.

CK2α1 NCBI Database Entry.

The CK2α1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

You are viewing: V4482 Change Configuration

SDS

Choose language:

Download SDSPDF (90 KB) – English (United States)

Certificate of Analysis

See all certificates for V4482

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V6287 Change Configuration

SDS

Choose language:

Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

See all certificates for V6287

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V4483 Change Configuration

What's in the box?

Item Part # Size
CK2α1 Kinase Enzyme System V4482 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

SDS

Choose language:

Download SDSPDF (213 KB) – English (United States)

Certificate of Analysis

See all certificates for V4483

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Let's find the product that meets your needs.

Talk to a Scientist

Sabine

Sabine

Germany