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CK1ε Kinase Enzyme System

Easily Screen and Profile CK1ε Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V4160

$ 449.00
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CK1ε Kinase Enzyme System
10µg
$ 449.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CK1ε Kinase Enzyme System contains:

  • CK1ε Kinase, 10μg (Human, recombinant full-length). MW: ~72kDa.
  • Casein, dephosphorylated, Substrate; native protein purified from bovine milk.
  • Reaction Buffer, DTT.

Full-length recombinant human CK1ε (CK1epsilon) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1ε is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes, including DNA replication and repair. CK1ε is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CK1ε is activated by CCK2R, and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CKIε can phosphorylate topoisomerase (topo) IIalpha at serine-1106, and this regulates the enzyme activity and sensitivity to topo II-targeted drugs.

CK1ε NCBI Database Entry.

The CK1ε Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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For Research Use Only. Not for Use in Diagnostic Procedures.

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What's in the box?

Item Part # Size
CK1ε Kinase Enzyme System V4160 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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