The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CK1γ1 Kinase Enzyme System contains:
- CK1γ1 Kinase, 10μg (Human, recombinant; amino acids 21–end). MW: ~70–76kDa.
- Native Casein Protein Substrate; purified from bovine milk.
- Reaction Buffer, DTT.
Recombinant human CK1 gamma 1 (21–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1γ1 or casein kinase 1, gamma 1 is one of the most abundant serine/threonine kinase in eukaryotic cells and is mainly involved in growth and morphogenesis. CK1γ1 possesses the C-terminal sequence motif (MTM), which it shares with CSNK1G2 and CSNK1G3; this motif is associated with heterologous carboxy-terminal sequences. CK1γ1 couples Wnt receptor activation to the cytoplasmic signal transduction apparatus.
CK1γ1 NCBI Database Entry.
The CK1γ1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.