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ABL1 (E255K) Kinase Enzyme System

V4492

Easily Screen and Profile ABL1 (E255K) Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V5098

$ 435.00 Your price: Log In

ABL1 (E255K) Kinase Enzyme System
10µg
$ 435.00
Your price: Log In
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The ABL1 (E255K) Kinase Enzyme System contains:

  • ABL1 (E255K) Kinase, 10μg (Human, recombinant; amino acids 27–end). MW: ~160kDa.
  • Abltide Substrate (EAIYAAPFAKKK); derived from the C-terminus of ABL.
  • Reaction Buffer, MnCl2, DTT.

Recombinant human ABL1 (E255K) (27–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ABL1 (E255K) is a mutant form of ABL1, a protooncogene encoding a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion and stress responses.

ABL1 (E255K) NCBI Database Entry.

The ABL1 (E255K) Kinase Enzyme System can be purchased with or without ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

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What's in the box?

Item Part # Size

ABL1 (E255K) Kinase Enzyme System

V5098 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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Patents and Disclaimers

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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