Features and Benefits
Objectively Quantify CPE: The assay provides quantifiable data by luminescence detection, which obviates subjective operator error associated with visual scoring methods.
Decrease Time to Results: Data can be recorded and analysis begun 10 minutes after reagent addition.
Simplify Assessment of CPE: The homogeneous “add-mix-measure” protocol dramatically reduces the manual steps required for CPE assessment.
Choose Your Format: The reagent is scalable from 96- to 1536-well plate formats.
Amenable to High Throughput Screening: Luminescent signal is very stable with a half-life generally >5 hours dependent on cell type and medium used, allowing batch or consecutive processing. No fluorescence interference results in high signal to background and delivers excellent Z′ values in screening applications.
Applications include the determination of viral infectivity and the corresponding tissue culture infective dose (TCID50
) and potential antiviral potency or off-target toxicity of test compounds.
This method is only useful for viruses that produce cytotoxicity and CPE.