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MultiTox-Fluor Multiplex Cytotoxicity Assay

A Fluorescent Assay That Measures Both Cell Viability and Cytotoxicity

  • Assay normalizes data for cell number
  • Reduces false-positive and false-negative results

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Catalog number selected: G9200

$ 276.00
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MultiTox-Fluor Multiplex Cytotoxicity Assay
10ml
$ 276.00
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Measure the Number of Live and Dead Cells Simultaneously

The single-reagent-addition, homogeneous, fluorescent assay measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. A second, cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity. 

The homogeneous, "add-mix-measure" protocol eliminates parallel plate processing and reduces cell culture costs, and the ratio of live:dead cells is independent of cell number and normalizes data. Data normalization for cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.

Complementary live- and dead-cell measures with independent chemistries serve as internal controls for each other, and multiplexing capability with most Promega bioluminescent cell-based apoptosis or genetic reporter assays results in more data per well.

The ratiometric response obtained from the MultiTox-Fluor Assay technology.

Notes

Cat # G9200 contains sufficient reagents for 100 assays at 100μl/assay in a 96-well format or 400 assays at 25μl/assay in a 384-well format. Cat # G9201 contains sufficient reagents for 500 assays at 100μl/assay in a 96-well format or 2,000 assays at 25μl/assay in a 384-well format. Cat # G9202 contains sufficient reagents for 1,000 assays at 100μl/assay in a 96-well format or 4,000 assays at 25μl/assay in a 384-well format.

Specifications

You are viewing: G9200 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 1 × 10μl

bis-AAF-R110 Substrate

G609A 1 × 10μl

Assay Buffer

G610A 1 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9201 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 5 × 10μl

bis-AAF-R110 Substrate

G609A 5 × 10μl

Assay Buffer

G610A 5 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9202 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608B 2 × 50μl

bis-AAF-R110 Substrate

G609B 2 × 50μl

Assay Buffer

G610B 2 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

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