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CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT)

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A Colorimetric Assay to Assess Cell Viability

  • Greater sensitivity than the neutral red procedure
  • Non-radioactive, fast and convenient assay applicable to a variety of cells

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Catalog number selected: G4000

$ 361.00
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CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT)
1,000 assays
$ 361.00
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The CellTiter 96® Non-radioactive Cell Proliferation Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems including: 1) serum protein precipitation caused by adding organic solvent; 2) interference by phenol red; 3) incomplete solubilization of the formazan crystals resulting in lower sensitivity; and 4) stability of the colored product.

The CellTiter 96® Assay is performed by adding a premixed, optimized Dye Solution to culture wells of a 96-well plate, usually containing various concentrations of growth factor or test substance. During a 4-hour incubation, living cells convert the MTT tetrazolium component of the Dye Solution into a formazan product. If you currently use a [3H]-thymidine incorporation assay, the addition of Dye Solution can be substituted for the pulse of radioactive thymidine at the time point in the assay when the pulse of [3H]-thymidine is usually added. The Solubilization/Stop Solution is then added to the culture wells to solubilize the formazan product, and the absorbance at 570nm is recorded using a 96-well plate reader. In addition, direct comparison between [3H]-thymidine incorporation and tetrazolium conversion have demonstrated less than a 5% difference between the two assays for determination of growth factor content of several samples.

Features and Benefits

Gain Sensitivity: Detect as few as 1,000 cells/well with a 96-well plate reader. Greater sensitivity than the neutral red assay procedure.

Use With a Variety of Cells: Assay mammalian, plant and yeast cells.

Non-Radioactive: Requires no scintillation cocktail or radioactive waste disposal.

Save Time: Perform the assay in a 96-well plate with no washing steps, no cell harvesting and no scintillation counting.

Convenient and Adapts to Your Needs: Requires no weighing or mixing of dye compounds and follows either a 4-hour or overnight protocol.


Specifications

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Whats in the box

Item Part # Size

Solubilization Solution/Stop Mix

G401A 1 × 100ml

Dye Solution

G402A 1 × 15ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Store Dye Solution at –20°C and Solubilization/Stop Solution at room temperature.

Specifications

You are viewing: G4100 Change Configuration

Whats in the box

Item Part # Size

Solubilization Solution/Stop Mix

G4101 1 × 500ml

Dye Solution

G4102 1 × 75ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Store Dye Solution at –20°C and Solubilization/Stop Solution at room temperature.

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