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Caspase-Glo® 8 Assay Systems

A Luminescent Assay That Measures Caspase-8 Activity

  • Decreased assay time and less enzyme needed
  • Homogeneous “add-mix-measure” protocol amenable to automation

Size

Catalog number selected: G8200

$ 138.00
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Caspase-Glo® 8 Assay Systems
2.5ml
$ 138.00
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A Homogenous, Luminescent Assay That Measures Caspase-8 Activity

The assay provides a proluminogenic caspase-8 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 8 Reagent in an "add-mix-read" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable "glow-type" luminescent signal and improves performance across a wide range of assay conditions.

The system now includes a separate vial of a protease inhibitor, MG-132 Inhibitor, which may be used to reduce background, thus improving the performance of the Caspase-Glo® 8 Assay in cell-based applications.

Features and Benefits

  • Low background luminescence results in excellent signal-to-noise ratios and superior sensitivity not achieved by other caspase formats, allowing assays to be performed in 96- or 384-well formats.
  • No sample preparation or manipulation required, and no extended incubation times are necessary as with fluorescent-based assays. Maximum sensitivity is achieved in as little as 0.5–1 hour.
  • Delivers excellent Z´ factors in cell and purified enzyme models.

Notes:  Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8200 provides sufficient reagents to perform 25 assays; Cat.# G8201, 100 assays; Cat.# G8202, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8200 provides sufficient reagents to perform 100 assays; Cat.# G8201, 400 assays; Cat.# G8202, 4,000 assays.

Sensitivity of the Caspase-Glo® 8 Assay in apoptotic Jurkat cells with MG-132 Inhibitor added to the Caspase-Glo® 8 Reagent.

Sensitivity of the Caspase-Glo® 8 Assay in apoptotic Jurkat cells with MG-132 Inhibitor added to the Caspase-Glo® 8 Reagent.
Sensitivity of the Caspase-Glo® 8 Assay in apoptotic Jurkat cells with MG-132 Inhibitor added to the Caspase-Glo® 8 Reagent.

Specifications

You are viewing: G8200 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 8 Substrate

G815C 1 × 1 bottle

Caspase-Glo® 8 Buffer

G817C 1 × 2.5ml

MG-132 Inhibitor

G932A 1 × 7.5μl

SDS

Choose language:

Download SDSPDF (159 KB) – English (United States)

Certificate of Analysis

See all certificates for G8200

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8201 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 8 Substrate

G815A 1 × 1 bottle

Caspase-Glo® 8 Buffer

G817A 1 × 10ml

MG-132 Inhibitor

G932B 1 × 30μl

SDS

Choose language:

Download SDSPDF (155 KB) – English (United States)

Certificate of Analysis

See all certificates for G8201

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8202 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 8 Substrate

G815B 1 × 1 bottle

Caspase-Glo® 8 Buffer

G817B 1 × 100ml

MG-132 Inhibitor

G932C 1 × 300μl

SDS

Choose language:

Download SDSPDF (159 KB) – English (United States)

Certificate of Analysis

See all certificates for G8202

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

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