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P450-Glo™ CYP1A1 Assay System

A Luminescent Method for Measuring CYP1A1 Activity

  • Luminescent format eliminates need for time-consuming analyses
  • Broad dynamic range and low background

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Catalog number selected: V8751

$ 145.00
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P450-Glo™ CYP1A1 Assay System
10ml
$ 145.00
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The P450-Glo™ CYP1A1 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP1A1 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.

This luminescent assay exhibits exquisite sensitivity, low background signals and broad dynamic range. It generates a "glow-type" luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The luminescent format also eliminates the need for time-consuming analyses such as HPLC.

The luciferin-based substrates are readily taken up by cells and rapidly converted into luciferin inside the cell, which reduces the incubation time required (typically 30–60 minutes). Other benefits of the P450-Glo™ CYP1A1 Assay include a formulation that minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors. Z´ values greater than 0.8 are achieved in either 96- or 384-well plate formats and confer highly predictive results.

Specifications

You are viewing: V8751 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859A 1 × 1 each

Luciferin-CEE, 5mM

V863A 1 × 70μl

Reconstitution Buffer

V865A 1 × 10ml

SDS

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Download SDSPDF (99 KB) – English (United States)

Certificate of Analysis

See all certificates for V8751

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

Specifications

You are viewing: V8752 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859B 1 × 1 each

Luciferin-CEE, 5mM

V863B 1 × 350μl

Reconstitution Buffer

V865B 1 × 50ml

SDS

Choose language:

Download SDSPDF (99 KB) – English (United States)

Certificate of Analysis

See all certificates for V8752

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

Resources

Citations

  • Evaluation of Luminogenic Substrates as Probe Substrates for Bacterial Cytochrome P450 Enzymes: Application to Mycobacterium tuberculosis.


    --
    2019 SLAS Discov
  • See all citations

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