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MAO-Glo™ Assay Systems

A Luminescent Method for Measuring Monoamine Oxidase (MAO) Activity

  • Luminescence format allows for fast results
  • No interference from fluorescence

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Catalog number selected: V1401

$ 145.00 Your price: Log In

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MAO-Glo™ Assay Systems
MAO-Glo Assay/200 assays
$ 145.00
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Measure MAO Activity From Recombinant and Native Sources

The MAO-Glo™ Assay is performed by incubating the MAO enzyme source with a luminogenic MAO substrate. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of MAO.

After the MAO reaction has been performed, the reconstituted Luciferin Detection Reagent is added. The reagent simultaneously stops the MAO reaction and initiates a stable glow-type luminescent signal with a half-life greater than 5 hours. This eliminates the need for strictly timed luminescent detection.

The MAO-Glo™ Assay includes a luminogenic MAO substrate, two MAO Reaction Buffers (one that can be used with either MAO A or MAO B enzyme and one that is designed specifically for MAO B), a lyophilized Luciferin Detection Reagent and the Luciferin Detection Buffer. The user supplies the sample material containing MAO. Protocols are configured for multiwell plate formats but easily can be adapted for single-tube applications.

The MAO-Glo™ Assay with MAO-A contains human recombinant MAO-A enzyme expressed in yeast. The kit is very well suited for the rapid assessment of potential inhibition of MAO-A by new chemical entities and can be used for higher throughput applications such as primary screening. The MAO-A enzyme is also available separately.

Features and benefits

  • Luminescence format eliminate the need for time consuming analyses such as HPLC.
  • Simple "add and read" protocol makes the assay amenable to high-throughput screening in multiwell plates.
  • Less MAO enzyme is required in these assays than in typical HPLC or fluorometric methods because of the enhanced sensitivity.

Protocols

Specifications

You are viewing: 200 assays Change Configuration

What's in the box?

Item Part # Size

MAO Substrate

V141A 1 x 186μg

MAO Reaction Buffer

V142A 1 x 10ml

MAO B Reaction Buffer

V143A 1 x 10ml

Reconstitution Buffer with esterase

V144A 1 x 10ml

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 1,000 assays Change Configuration

What's in the box?

Item Part # Size

MAO Substrate

V141B 1 x 931μg

MAO Reaction Buffer

V142B 1 x 50ml

MAO B Reaction Buffer

V143B 1 x 50ml

Reconstitution Buffer with esterase

V144B 1 x 50ml

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 500μl Change Configuration

What's in the box?

Item Part # Size

MAO-A

V145A 1 x 500μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: 1,000 assays Change Configuration

What's in the box?

Item Part # Size

MAO Substrate

V141B 1 x 931μg

MAO Reaction Buffer

V142B 1 x 50ml

MAO B Reaction Buffer

V143B 1 x 50ml

Reconstitution Buffer with esterase

V144B 1 x 50ml

MAO-A

V145A 1 x 500μl

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

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