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PowerPlex® 21 System

Multiplex STR System for Human Identification

  • Increases discriminatory power and data-sharing possibilities with loci commonly used in US, Europe and Asia
  • Optimized protocols for many sample types, including direct amplification from FTA® cards and pretreated non FTA® card punches or swabs
  • Rapid cycling technology reduces thermal cycling time up to 2 hours

Size

Catalog number selected: DC8902

$ 4,892.00 Your price: Log In

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PowerPlex® 21 System
200 reactions
$ 4,892.00
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Fast Process and Flexible Protocols

The PowerPlex® 21 System is a multiplex STR system for human identification applications including forensic analysis, relationship testing and research use. The system allows co-amplification and fluorescent detection of 21 loci (20 STR loci and Amelogenin), including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA. The system offers high discrimination with 21 markers for difficult cases and complete data overlap with most existing multiplexes.

The PowerPlex® 21 System increases laboratory productivity and decreases average turnaround time with 90-minute PCR. Laboratories can also save labor and time since the wash step for FTA® cards is eliminated. Simpler protocols are available for swabs and nonFTA card punches as well. The higher inhibitor tolerance of this system benefits challenging casework samples and results in less locus drop-out and reaction failure for higher success rates.

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A single-source template DNA (0.5ng) was amplified using the PowerPlex® 21 System and analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. Results were analyzed using GeneMapper® ID software, version 3.2.
ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Flexibility for Most Sample Types

The PowerPlex® 21 System is designed to work with a variety of sample types, including difficult casework samples and is compatible with direct amplification from FTA® card punches as well as pretreated swabs and Bode Buccal Collector™ device or S&S 903 punches.

Genomic DNA
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Amplification of 500pg of human genomic DNA using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection).
Blood on FTA® Card
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Direct amplification of one 1.2mm punch from blood on an FTA® card using recommended protocol. Amplified products were separated on Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection). 
Buccal Samples on S&S 903
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A 1.2mm punch from buccal samples on S&S 903 was pre-treated with SwabSolution™ Reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection). 

Swab from Buccal Samples

10370TA-W
A swab from buccal samples was pre-treated with SwabSolution™ Reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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Protocols

Specifications

You are viewing: DC8902 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 x 25μl10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 1 x 200μl View Product
PowerPlex® 21 Allelic Ladder Mix DG247B 1 x 100μl
PowerPlex® 21 5X Primer Pair Mix DK183B 1 x 1ml
PowerPlex® 21 5X Master Mix DP107A 1 x 1ml

Water, Amplification Grade

DW099A 5 x 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. No. 6,238,863 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC8942 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 4 x 25μl10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 4 x 200μl View Product
PowerPlex® 21 Allelic Ladder Mix DG247B 4 x 100μl
PowerPlex® 21 5X Primer Pair Mix DK183B 4 x 1ml
PowerPlex® 21 5X Master Mix DP107A 4 x 1ml

Water, Amplification Grade

DW0991 4 x 6,250μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. No. 6,238,863 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

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