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PowerPlex® Fusion System

Co-Amplification and Fluorescent Detection of 24 Loci

  • Meets CODIS and European standards
  • High inhibitor tolerance and sensitivity for casework
  • One kit for both casework and database sections

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Catalog number selected: DC2402

$ 4,651.00
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PowerPlex® Fusion System
200 reactions
$ 4,651.00
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More Information and Superior  Performance from a Single Kit

The PowerPlex® Fusion System provides all of the materials needed for co-amplification and fluorescent detection of 24 loci (23 STR loci and Amelogenin, but without SE33), including the CODIS core loci and European Standard Set (ESS) loci. This comprehensive set supports compatibility with present databases across multiple regions and encompasses an expanded set of loci to address future growth. The PowerPlex® Fusion System delivers more information and high success rates from demanding forensic, paternity and relationship-testing cases. Using proven STR chemistries for existing instrument platforms and software, this 5-color system requires no software or instrument upgrades.

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

High Discriminatory Power

PowerPlex® Fusion loci set is exceptional. By combining US CODIS and ESS loci, the system offers significant gains in discriminatory power and helps ensure fewer adventitious matches are obtained.

A probability of identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus.
STR Kit Number of Loci PI (N = 1036)
CODIS 13
13
5.02 × 10–16
ESS 12
12
3.04 × 10–16
CODIS 20
20
9.35 × 10–24
GlobalFiler™ STR Kit
23
7.73 × 10–28
Investigator® 24plex Kit
23
7.73 × 10–28
PowerPlex® Fusion System
24
6.58 × 10–29
PowerPlex® Fusion 6C System
27
2.30 × 10–32

Note: PI values do not include Amelogenin or Y-STRs.

Reference: Butler, J.M., Hill, C.R. and Coble, M.D. Variability of new STR loci and kits in US population groups. Profiles in DNA (www.promega.com/resources/profiles-in-dna/2012/variability-of-new-str-loci-and-kits-in-us-population-groups/). Published 2012. Accessed April 2012.

Maximum Sensitivity

The PowerPlex® Fusion System reliably produces complete STR profiles from as little as 100pg of human DNA
10911MA-W
Amplification of 100pg of human DNA using 30 cycles and the PowerPlex® Fusion System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5‑second injection).
14098MA-W
The PowerPlex® Fusion System demonstrates superior sensitivity, generating full profiles with as little as 100pg input DNA.

Superior Inhibitor Tolerance

With a robust buffer system designed to tolerate the most challenging inhibitors, the PowerPlex® Fusion System minimizes the need to re-amplify samples generally thought to be too challenging to run, saving your lab countless hours of repeat analyses.

14099MA-W
Human genomic DNA (500pg) was amplified with each listed kit in the presence of common PCR inhibitors.

Direct-Amplification Compatibility for Most Sample Types

Building on the proven flexibility of our PowerPlex® 21 System, the PowerPlex® Fusion System allows direct amplification from FTA® card punches as well as pretreated nonFTA cards and commonly used swabs to streamline workflow and expand sample throughput.
Buccal Punch Card
10913MA-W
One 1.2mm buccal punch of an S&S 903 card was pretreated with PunchSolution™ Reagent
Swab
10914MA-W
A swab was pretreated with SwabSolution™ Reagent. Following incubation, 2μl of extract was added to the PCR amplification mix and amplified.
FTA® Card Punch with Blood
10915MA-W
Direct amplification of one 1.2mm FTA® card punch containing blood.
Buccal Punch Card
10915MA-W
Direct amplification of one 1.2mm FTA® card punch containing blood.

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

Which Product is Right for Me?

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Want to Automate?

Want to spend more time on STR data analysis? Let the STR Normalization Manager Software set up your PCR amplifications using the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan) or MicroLab STAR (Hamilton) liquid handlers. The software uses quantitation data from the PowerQuant® or Plexor® HY Systems to accurately dilute and set up STR amplification of extracted DNA. The software can also set up direct amplification from swabs and card punches.

Automate Your STR Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Consumables
Product Size Cat. #
1.1ml, Square-Well V-bottom Deep Well Plate
25/pack
2.2ml, Square-Well Deep Well Plate
50/pack
Water, Amplification Grade
6,250ml
Hardware
Product Cat. #
Four-Position Tube Holder
Heat Block Adapter
Plate Clamp 96
Plate Stand
Software
Product Cat. #
STR Normalization Manager Software

Specifications

You are viewing: DC2402 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 1 × 200μl View Product
PowerPlex® Fusion Allelic Ladder Mix DG381B 1 × 100μl

PowerPlex® Fusion 5X Primer Pair Mix

DK236B 1 × 1ml
PowerPlex® Fusion 5X Master Mix DP207A 1 × 1ml

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (163 KB) – English (United States)

Certificate of Analysis

See all certificates for DC2402

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,238,863 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC2408 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 4 × 200μl View Product
PowerPlex® Fusion Allelic Ladder Mix DG381B 4 × 100μl

PowerPlex® Fusion 5X Primer Pair Mix

DK236B 4 × 1ml
PowerPlex® Fusion 5X Master Mix DP207A 4 × 1ml

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (163 KB) – English (United States)

Certificate of Analysis

See all certificates for DC2408

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,238,863 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

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