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High Specificity, High Capacity Magnetic Affinity Beads for Antibody Capture

  • Binding capacities of >25mg/ml depending on antibody species, isotype
  • Minimize purification losses and increase sample throughput with exceptional magnetic properties

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Choose Protein G or A Beads

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$ 145.00

Catalog Number: G7471

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$ 558.00

Catalog Number: G7472

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$ 4,188.00

Catalog Number: G7473

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$ 146.00

Catalog Number: G8781

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$ 552.00

Catalog Number: G8782

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Overview
Protocols
Specifications
Resources
Related Products
Citations

Magnetic Protein A and G Antibody Purification

High-yield, robust purification of antibodies from serum, ascites and cell media.

(Note: Magne® Protein G and Magne® Protein A Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.)

Antibody Purified from Various Sample Types using the Magne® Protein A and Magne® Protein G Beads.

11208TA-W
Antibody was purified from 50μl of cell culture media (mouse IgG1), mouse ascites (IgG2a) and goat serum with 50μl of Magne® Protein A Beads (A) and Magne® Protein G Beads (G) using published protocols (Technical Manual #TM371). Samples were separated via SDS-PAGE by adding 1μl of starting material (SM) or 5μl of purified sample (A or G) and stained with Coomassie®-based stain

Schematic of Antibody Purification using Magne® Protein A Beads or Magne® Protein G Beads.

11207TA-W

Competitor Comparison for Magnetic Antibody Purification Resins

10751MA-W
Comparison of antibody yield using the Magne® Protein G Beads versus competitors' products from diverse sample types. *Delipidated human gamma globulin serum fraction was used as the source material.

Relative Binding Affinities of Protein A and Protein G for Different Antibody Species and Isotypes.

Species/ Subclass Protein A Protein G
Monoclonal
Human
IgG1 +++ +++
IgG2 +++ +++
IgG3 + +++
IgG4 +++ +++
Key: Strong (+++), Medium (++), Weak (+). Adapted from Akerström, B. et al. (1985) J. Immunol. 135(4), 2589-92.
Species/ Subclass Protein A Protein G
Monoclonal
Mouse
IgG1 + +++
IgG2a +++ +++
IgG2b ++ ++
IgG3 + ++
Species/ Subclass Protein A Protein G
Monoclonal
Rat
IgG1 + +
IgG2a + +++
IgG2b + ++
IgG2c + +
Species/ Subclass Protein A Protein G
Polyclonal
Rabbit +++ +++
Cow ++ +++
Goat + +++
Chicken + +
Human IgG +++ +++
Human IgM + +
Human IgD + +
Human IgA + +

Specifications

Catalog Number:

What's in the box?

Item Part # Size

Magne® Protein G Beads

G747A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

What's in the box?

Item Part # Size

Magne® Protein G Beads

G747A 5 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

What's in the box?

Item Part # Size

Magne® Protein G Beads, 20% Slurry

G747B 1 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878A 5 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

What's in the box?

Item Part # Size

Magne® Protein A Beads, 20% Slurry

G878B 1 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

U.S. Pat. No. 10,604,745.

Product Citations

Recent publications that mention the use of this product.

View PubMed Citations