Part One: Designing the Experiment
Part Two: Lab Work
Study Proteins Under Endogenous Regulation
Using CRISPR/Cas9 gene editing, HiBiT-tagged proteins can be expressed under endogenous regulatory conditions, reducing overexpression artifacts and maintaining proper stoichiometry with other endogenous binding partners or regulatory machinery.
The small 11 aa HiBiT tag can be added to the desired protein in one of two ways:
- Traditional recombinant methods with your promoter of choice
- Insertion into endogenous loci using a simple, clone-free CRISPR/Cas9 genome editing protocol