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Applitek EZ-ATP® Online Analyzer

Total Solution for Online Microbial Analysis of Water Samples

  • Complies with ASTM D4012 Standard Method
  • Measures 0.5–200pg/ml ATP
  • Low cost of analysis at 2,500 measurements per 250ml bottle of reagent

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This product is discontinued
Applitek EZ-ATP® Online Analyzer

Online ATP Monitoring Protects Processes

 No Delay between sample collection, measurement, analysis and reporting.

 Rapid measurement: 10–15 minutes (including lysis of sample).

 Auto calibration and built-in cleaning protocol.

 Stable reagent (6 months at 4°C).

 Eliminates bias from the composition of growth media, as seen with plate counting.

 Complete ATP recovery: Detect intracellular, extracellular and total ATP.


Rapidly Assess Microbial Contamination from Industrial Process or Drinking Water

Every process engineer knows that bacteria present in water samples cause issues such as biofilm formation and microbiologically induced corrosion (MIC). Measuring ATP levels in water samples can be easily correlated to viable biomass (microbes present). Combining the sensitivity and reliability of ATP detection with online sampling and analysis of process and drinking water creates a powerful method for rapid and sensitive detection of microbial detection in water samples, allowing you to intervene quickly and prevent downtime.


Benefits of the EZ-ATP® Analyzer with Water-Glo™ Chemistry

 Monitor contamination to prevent biofouling

 Reduce biofilm formation on surfaces

 Limit microbiologically-induced corrosion (MIC)

 Monitor health of biofiltration beds

 Extend lifetime of membranes by preventing clogging

 Monitor effectiveness of biocide treatments

Linear Response of Entire Analytical Range


Monitor Low Levels for Sensitive Applications


Frequently Asked Questions

What is ATP and how can you use it to measure bacteria?

ATP is the “energy currency” of all living cells and degrades rapidly when an organism dies. Because of this property, ATP serves as a biomarker for living bacteria in a sample and can be used to quantitate the presence of live bacteria. 

What is bioluminescence?

Many living organisms, including fireflies, release light energy from chemical reactions. These reactions are catalyzed by proteins in the cell called luciferases, and they require oxygen, ATP and a luciferase substrate. The bioluminescent reaction can be harnessed to detect the presence of the required substrates (such as ATP) in the environment.

Does EZ-ATP® identify the type of bacteria present in a water sample?

No. The system identifies the presence of living organisms, but does not identify species. It can be used as an early detection system, and if an ATP signal is detected, other methods, such as PCR or NGS, may be used to identify the specific organism.

How does ATP measurement compare to other forms of microbial detection?

The most common methods of microbial detection in water measure biofouling potential (TDC, AOC or BDOC) or the development of biofouling (pressure drop or BFR). Heterotrophic plate counting does directly measure viable microbes, but it is slow and detects less than 10% of cultivable bacteria. Flow cytometry and PCR improve turnaround time and accuracy, but neither method directly measures live bacteria. ATP detection is the only method that directly assesses living cells from any type of microbe, providing an immediate indication of microbial contamination in your sample.

To request a quote or get more information about the
Applitek EZ ATP Online, contact applied@promega.com

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