The ADP-Glo™ Kinase Assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.
The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling. Z'-Factors >0.7 are routinely obtained. The stable luminescent signal allows batch plate processing without the need for strictly timed incubations.