Luciferases are commonly used to monitor gene expression because of their broad dynamic range and extreme sensitivity. The dual-luciferase assay format is widely used to reduce experimental variability by comparing the response of the experimental reporter to that of a constitutively expressed control. In this way, variability arising from well-to-well or experiment-to-experiment differences in parameters such as transfection efficiency and cell number can be reduced or eliminated.
This webinar introduces the next-generation dual-luciferase assay, Nano-Glo® Dual-Luciferase® (NanoDLR™) Reporter Assay System, combining firefly and NanoLuc® Luciferases. The new assay offers the experimental freedom of the Dual-Glo® Reporter Assay System with regard to signal half-life and the brightness of the Dual-Luciferase® Reporter Assay System.
The webinar also discusses the concept of the coincidence reporter assay, an assay developed at the NIH that is especially useful for monitoring reporter activity due to promoter activation in screening applications. The coincidence reporter system is designed to identify false hits more rapidly so time and money are not wasted.
Christopher T. Eggers, PhD
Senior Research Scientist
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