| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
SL-2 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.9 |
3:1 |
2005. Drug Metab. Dispos. 33: 1244–53 |
Description of cell line: Drosophila Schneider cell line
Additional Relevant Information: DNA and Fugene 6 were mixed in Hanks' balanced salt solution and incubated at room temperature for 30 min before addition to the cells.
Type of Transfection:
|
 |
ST2 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
2002. J. Biol. Chem. 277: 26217-24 |
Description of cell line: mouse bone marrow stroma
Additional Relevant Information: cells grown to 50-80% confluence prior to transfection
Type of Transfection:
|
|
SV-589 |
FuGENE HD Transfection Reagent |
DNA |
35mm dish |
2 |
3:1 |
2012. Mol. Biol. Cell 23: 864–80 |
Description of cell line: Immortalized Human Fibroblast Line
Additional Relevant Information:
Type of Transfection:
|
|
SW962 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
6:1 |
2003. Nat. Cell Biol. 5: 711–9 |
Description of cell line: squamous cell carcinoma
Additional Relevant Information: plated at 50% confluency 16hr prior to transfection
Type of Transfection:
|
|
TCMK |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2000. J. Virol. 74: 5776–87 |
Description of cell line: mouse kidney
Additional Relevant Information: 2x10e5 to 4x10e5 cells plated 24hr prior to transfection
Type of Transfection:
|
|
THP-1 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
5 |
2:1 |
|
Description of cell line: human monocytic cell line
Additional Relevant Information: cells in suspension at 1x10e6 cells/ml
Type of Transfection:
|
|
|
|
|
|
|
|
|
Description of cell line:
Additional Relevant Information:
Type of Transfection:
|
|
U2OS |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
5 |
3:1 |
2001. J. Virol. 75: 9142–55 |
Description of cell line: human osteosarcoma cell line
Additional Relevant Information: stable cell-line generated using puromycin selection
Type of Transfection:
|
|
U2OS |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.45 |
3:1 |
2000. Proc. Natl. Acad. Sci. USA 97: 13275-80 |
Description of cell line: human osteosarcoma cell line
Additional Relevant Information: cells plated at 5x10e5 cells/well
Type of Transfection:
|
|
U2OS |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
not reported |
3:1 |
2003. J. Cell Sci. 116: 1319–1326 |
Description of cell line: human osteosarcoma cell line
Additional Relevant Information:
Type of Transfection:
|
|
U-87MG |
FuGENE HD Transfection Reagent |
DNA |
100mm dish |
10 |
5:2 |
2009. Proc. Natl. Acad. Sci. USA 106: 480–485 |
Description of cell line: Human glioblastoma astrocytoma
Additional Relevant Information:
Type of Transfection:
|
|
U937 |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
10 |
5:1 |
1999. J. Exp. Med. 190: 1679–1688 |
Description of cell line: human leukemic monocyte lymphoma cell line
Additional Relevant Information: 3x10e6 cells/plate
Type of Transfection:
|
|
U937 |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
2.155 |
not reported |
2011. J. Virol. 85: 10719-29 |
Description of cell line: human leukemic monocyte lymphoma cell line
Additional Relevant Information:
Type of Transfection:
|
|
Vero |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
1 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: African green monkey kidney cell line
Additional Relevant Information: 5x10e4 cells plated per well
Type of Transfection:
|
|
Vero |
FuGENE HD Transfection Reagent |
DNA |
60mm dish |
3.5 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: African green monkey kidney cell line
Additional Relevant Information: 4x10e5 cells per plate
Type of Transfection:
|
|
Vero |
FuGENE HD Transfection Reagent |
DNA |
not reported |
2 |
6:1 |
2012. J. Virol. 86: 1758–67 |
Description of cell line: African green monkey kidney cell line
Additional Relevant Information: 10e6 cells transfected
Type of Transfection:
|
|
WM266.4 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
6:1 |
2003. Nat. Cell Biol. 5: 711–9 |
Description of cell line: melanoma
Additional Relevant Information: plated at 50% confluency 16hr prior to transfection
Type of Transfection:
|
|