| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
Hep-2 |
FuGENE HD Transfection Reagent |
DNA |
24-well |
1 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: HeLa derivative
Additional Relevant Information: 5x10e4 cells plated per well
Type of Transfection:
|
 |
Hep-2 |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
0.5 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: HeLa derivative
Additional Relevant Information: 5x10e4 cells per well on glass coverslips
Type of Transfection:
|
|
HepG2 |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
1 |
3:1 |
2005. J. Lipid Res. 46: 2151–67 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: 2x10e5 cells plated/well, transfection mix included 0.05ug of control vector
Type of Transfection:
|
|
HepG2 |
FuGENE 6 Transfection Reagent |
DNA |
96-well plate |
0.2 |
3:1 |
2005. Drug Metab. Dispos. 33: 1244–53 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
HepG2 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
0.6 |
3:1 |
2004. J. Biol. Chem. 279: 13677-88 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: cells plated at 4x10e4
Type of Transfection:
|
|
HepG2 |
FuGENE 6 Transfection Reagent |
DNA |
T25 Flask |
2.5 |
3:1 |
2008. J. Biol. Chem. 283: 30650–7 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: mixed plasmids, 3 x10e6 cells (0-80% confluency)
Type of Transfection:
|
|
HepG2 |
FuGENE HD Transfection Reagent |
DNA |
150mm dish |
15 |
7:2 |
2013. Genome Res. 23: 800–11 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: 5x10e6 cells seeded per dish 1 day before transfection
Type of Transfection:
|
|
HepG2 |
FuGENE HD Transfection Reagent |
DNA |
150mm dish |
15 |
7:2 |
2013. Genome Res. 23: 800-11 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: cells plated at 5x10e6 cells/dish
Type of Transfection:
|
|
HepG2 |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2008. J. Biol. Chem. 283: 8984–94 |
Description of cell line: human liver hepatocellular carcinoma
Additional Relevant Information: 2x10e5 cells seeded onto coverslips 1 day prior to transfection
Type of Transfection:
|
|
hESC |
FuGENE HD Transfection Reagent |
DNA |
not reported |
2ug |
3:1 |
2011. Nucl. Acids Res. 39: e107 |
Description of cell line: Human embryonic stem cells
Additional Relevant Information: 2 × 10e6 hESCs were transfected 4 days following subculture
Type of Transfection:
|
|
HMC-1 |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
0.5 |
4:1 |
2010. FASEB J. 24: 4047–57 |
Description of cell line: Human mast cell line
Additional Relevant Information: 1x10e5 cells plated per well
Type of Transfection:
|
|
HT1080 |
FuGENE 6 Transfection Reagent |
DNA |
100mm dish |
5 |
3:1 |
2009. J. Biol. Chem. 284: 24306–24319 |
Description of cell line: fibrosarcoma epithelial cell
Additional Relevant Information:
Type of Transfection:
|
|
HUVEC |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.55 |
4:1 |
2003. J. Biol. Chem. 278: 6976-84 |
Description of cell line: human umbilical vein endothelial cells
Additional Relevant Information: cells plated at 1X10e5 cells/well 24hr prior to transfection
Type of Transfection:
|
|
Jurkat |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
0.6 |
3:1 |
2004. J. Biol. Chem. 279: 13677-88 |
Description of cell line: T lymphocyte
Additional Relevant Information: cells plated at 1x10e6, Jurkat clone E6.1
Type of Transfection:
|
|
Jurkat |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
0.25 |
6:1 |
2009. J. Immunol. 183: 7362–70 |
Description of cell line: T lymphocyte
Additional Relevant Information: 1x10e6 cells
Type of Transfection:
|
|
K562 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.2 or 0.3 |
3:1 |
2008. J. Biol. Chem. 283: 8984–94 |
Description of cell line: human chronic myelogenous leukemia
Additional Relevant Information:
Type of Transfection:
|
|
LBT2 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.275 |
3:1 |
2003. Endocrinology 144: 4410–9 |
Description of cell line: pituitary tumor, LHβ promoter linked to SV40T antigen oncogene
Additional Relevant Information: cells plated at 5x10e5 cells/well
Type of Transfection:
|
|
LS174T |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
6:1 |
2003. Nat. Cell Biol. 5: 711–9 |
Description of cell line: colon carcinoma
Additional Relevant Information: plated at 50% confluency 16hr prior to transfection
Type of Transfection:
|
|
MCF7 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.25 |
3:1 |
2008. Mol. Pharmacol. 73: 769-777 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information:
Type of Transfection:
|
|
MCF7 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
2003. Mol. Cancer Res. 1: 475-84 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information: Cells transfected at 70% confluency
Type of Transfection:
|
|