| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
CHO-K1 |
FuGENE HD Transfection Reagent |
DNA |
100mm dish |
6 |
3:1 |
2010. J. Biol. Chem. 285: 19288–98 |
Description of cell line: Chinese hamster ovary variant
Additional Relevant Information:
Type of Transfection:
|
 |
Chorionic |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
1.7 |
2:1 |
1998. J. Biol. Chem. 273: 25292-300 |
Description of cell line: human chorionic cells
Additional Relevant Information:
Type of Transfection:
|
|
CHO-7 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
3 |
3:1 |
2004. J. Biol. Chem. 279: 43136-47 |
Description of cell line: Chinese hamster ovary variant
Additional Relevant Information:
Type of Transfection:
|
|
CHO-K1 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2.4 |
3:1 |
2006. Am. J. Physiol. Endocrinol. Metab. 291: E704–15 |
Description of cell line: Chinese hamster ovary variant
Additional Relevant Information: Cells plated at 2x10e4 cells/well one day prior to transfection
Type of Transfection:
|
|
CHO-T |
FuGENE 6 Transfection Reagent |
DNA |
150 mm dish |
7.5-15 |
6:1 |
2003. J. Biol. Chem. 278: 11359-68 |
Description of cell line: Chinese hamster ovary variant
Additional Relevant Information: Cells transfected at 50-70% confluency
Type of Transfection:
|
|
COS-7 |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.23 |
2:1 |
1998. J. Biol. Chem. 273: 25292-300 |
Description of cell line: vervet monkey kidney fibroblast
Additional Relevant Information:
Type of Transfection:
|
|
COS-7 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
2.05 or 4.05 |
3:1 |
|
Description of cell line: vervet monkey kidney fibroblast
Additional Relevant Information: 5X10e5 cells plated, transfected at 50-60% confluency
Type of Transfection:
|
|
COS-7 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
8.25 |
3:2 |
2009. RNA 15: 686-95 |
Description of cell line: vervet monkey kidney fibroblast
Additional Relevant Information: cells grown to 80-90% confluency
Type of Transfection:
|
|
COS-7 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
6 |
1.67:1 |
2003. J. Exp. Med. 198: 557–67 |
Description of cell line: vervet monkey kidney fibroblast
Additional Relevant Information: cells plated at 5x10e5/plate 24hr prior to transfection
Type of Transfection:
|
|
DLD-1 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
not reported |
3:1 |
2006. J. Biol. Chem. 281: 14700–14710 |
Description of cell line: human epithelial colorectal adenocarcinoma
Additional Relevant Information: 4 × 10e4 cells/well
Type of Transfection:
|
|
Fibroblast |
FuGENE 6 Transfection Reagent |
DNA |
35 mm dish |
1 |
3:1 |
2003. Biol. Reprod. 69: 1060–1068 |
Description of cell line: primary fibroblast
Additional Relevant Information: 80% confluency, primary pig fetal fibroblast
Type of Transfection:
|
|
H4 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
2000. J. Biol. Chem. 275: 26277–26284 |
Description of cell line: human neuroglioma
Additional Relevant Information: 70% confluent cells, 800ug/ml Hygromycin used for stable cell selection
Type of Transfection:
|
|
HBL-100 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
0.5-2 |
not reported |
2000. J. Biol. Chem. 275: 38589–38596 |
Description of cell line: human breast epithelial cell line
Additional Relevant Information: 50-70% confluency, cells were plated on glass coverslips
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
not reported |
3:1 |
2011. Mol. Endocrinol. 25: 2144-56 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 50-60% confluency
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
35 mm dish |
4 |
3:2 |
|
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information:
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
1.2 |
3:1 |
2008. J. Gen. Virol. 89: 1978–86 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information:
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
2.05 and 4.05 |
3:1 |
|
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 5X10e5 cells plated, transfected at 50-60% confluency
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
2003. J. Biol. Chem. 278: 6787-6794 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: Cells plated at 1.5x10e4/well
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
0.25 |
6:1 |
2009. J. Immunol. 183: 7362–70 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 5x10e5 cells
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
T75 Flask |
not reported |
not reported |
2008. J. Endocrinol. 197: 95–109 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 3×10e6 cells/75 cm; 60% cell confluency; 60-70% efficiency
Type of Transfection:
|
|