Notes: CHO cells stably transfected with the PSD-95/SAP90 protein were generated using the pCI-Neo Mammalian Expression Vector. These stably transfected cells or standard CHO cells were transiently transfected with various eukaryotic expression constructs including some in the pCI-neo Vector. Transient transfection of the CHO cells was performed with the TransFast™ Reagent. (1267)

Notes: The TNT^® Coupled Wheat Germ Extract and the TNT^® Coupled Rabbit Reticulocyte Lysate Systems were used to in vitro express and ³⁵S-methionine label various Huntington protein mutants and polyglutamine-containing proteins. These proteins included a full-length 210KDa Huntington, a 210KDa Atrophin-1, and a 140KDa Androgen Receptor protein. The expressed proteins were incubated with apoptotic extracts or purified caspases to detect cleavage by caspases. Mutated Huntington constructs were cloned into the pCI-neo Vector.  Data from autoradiographs were quantitated by laser band densitometry. (3019)

Notes: The pCI-neo Mammalian Expression Vector was used to express the 22kDa caveolin I protein in L1210-JF lymphoid cells, which lack the caveolin protein. The expressed protein formed a chaperone complex just like NIH3T3 cells, which naturally express the protein. (0251)

Notes: The cDNA for the 35kDa Hakata antigen was subcloned into the pCI-neo Mammalian Expression Vector and transiently expressed in the PLC human liver cell line. Transfection was performed with Tfx™-50 Reagent. (0312)

Notes: Cloned carbonic anhydrase II gene into the pCI-neo Mammalian Expression System and used in gene therapy to correct deficiency in renal tubules of carbonic anhydrase-deficient mice. (0872)

Notes: C-terminally truncated versions of c-rel were synthesized using the TNT® Coupled Reticulocyte Lysate System. Proteins were purified away from unincorporated methionine using Centricon-30 ultrafiltration membranes. The pCI-neo Mammalian Expression Vector was used to express chicken c-rel in NIH3T3 and COS cells. (1322)

Notes: Used Dual-Luciferase^® Reporter Assay System with a 400:1 transfection ratio of the firefly luciferase plasmid to pRL-SV40 Vector. Also used the pCI-neo Mammalian Expression Vector to make several E1A expression plasmids. (0743)

Notes: The 903 nucleotide geranylgeranyl diphosphate synthase cDNA was cloned into the pCI-neo Mammalian Expression Vector and transiently expressed in CHO cells. (1223)

Notes: This paper describes the use of the pCI-neo Mammalian Expression Vector to overexpress human insulin receptor in βTC6-F7 β cells. (0123)

Notes: The pCI-neo Mammalian Expression Vector was used to express various mutant and wildtype tissue inhibitor of metalloproteinases (TIMP)-2 and TIMP-3 molecules in COS-7 cells. The 24-27kDa proteins were used to assess the function of the c-terminal and n-terminal domains for their metalloproteinase inhibitory functions and extracellular matrix binding functions. (0837)

Notes: The authors used pCI-neo Mammalian Expression Vector to express HA-tagged PPX in 293T cells. (1021)

Notes: The TERT protein with a hemagglutinin tag was expressed in 293 cells and GM847 SV40-transformed human fibroblasts using the pCI-Neo Mammalian Expression Vector. (1282)

Notes: The pCI-neo Mammalian Expression Vector was used to express the α subunit (>104kDa) of the high conductance Ca²⁺-activate K⁺ channel as well as various mutants of the β subunit (~30kDa) in COS-1 cells and TsA-201 cells (HEK 293 cells transformed with the SV40 T antigen). (1062)

Notes: The authors used Promega's pCI Mammalian Expression Vector to create several expression constructs. (0398)

Notes: The inhibitor was used to protect RNA during RT-PCR. (1671)

Notes: The pCI-Neo Mammalian Expression Vector was used to express a truncated env protein in THP-1 cells. (2014)

Notes: The cDNA for α-catenin was subcloned into the pCI-neo Mammalian Expression Vector, and a synthetic 6XHis-Tag was added to the cDNA. A deletion mutation of α-catenin was also subcloned into the vector. The His-tag was used to bring down complexes of the proteins, which were then probed on Western blots for the individual proteins. (1031)

Notes: The pCI-Neo Mammalian Expression Vector was used to express wildtype and mutant glucagon receptors either transiently (COS cells) or stably (CHO cells). The expressed protein was used for numerous assays of receptor function and receptor:ligand interactions. (1338)

Notes: The vector is used to express a human Neuropeptide Y-GFP fusion protein in PC12 cells. (1583)

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

Notes: The pCI-Neo Mammalian Expression Vector was used to express the prion PrP in mouse N2a cells. (0647)

Notes: The cholecystokinin (CCKb) receptor was expressed in COS-7 cells using the pCI-neo Mammalian Expression Vector. The cells were used for cell surface ligand binding studies. The Transfectam® Reagent was used to transiently transfect Jurkat T cells. Many details of the transfection are provided. (0597)

Notes: The pCI-neo Mammalian Expression Vector was used to construct a chimeric vector with the neo resistance gene downstream of an internal ribosome entry site, so that the neo gene was transcribed with the Cig30 cDNA and both protein controlled by the CMV promoter. The construct was used to make stable transfectants of the HIB-1B hibernoma cells. Cell lines were analyzed by Northern blotting. The protein was also analyzed in vitro with the Rabbit Reticulocyte Lysate and Canine Pancreatic Microsomal Membranes (CMMs). (0246)

Notes: The Kv1.1 and Kv1.2 cDNAs were each cloned into the pCI-neo Mammalian Expression Vector. The two ~80kDa proteins were transiently expressed in COS-1 cells and assayed for charybdotoxin and α-dendrotoxin binding as well as recognition by antisera. (0914)

Notes: Both sense and antisense bcl-2 and cytochrome P4502E1 cDNAs were stably expressed in a HepG2 derived cell line using the pCI-Neo Mammalian Expression Vector. Cytotoxicity of arachidonic acid to transfected and control cells was assessed using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay. (1328)