Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Citations Search

Sort By:

Genetics 154, 1239-1253. The Drosophila melanogaster ade5 gene encodes a bifunctional enzyme for two steps in the de novo purine synthesis pathway 2000

O'Donnell1, A. , Tiong, S., Nash, D. , Clark, D.

Notes: Polyadenylated RNA [poly(A)-RNA] was purified from 0.5 mg total RNA using the PolyATtract® mRNA Isolation System. For primer extension, a primer was radiolabeled by 5' phosphorylation with [γ-32P]ATP using the Primer Extension System. The primer extension reaction was performed using 10 µg of total RNA from adult Canton-S flies and 100 fmol of a 32P-labeled primer. Size markers were a 32P-labeled Promega phiX174/Hinf I Marker standard. (0588)

Expand Full Notes »