PolyATtract® mRNA Isolation Systems

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Isolates Highly Pure mRNA from Total RNA

  • Protocol complete in approximately 45 minutes
  • Isolated mRNA is suitable for use in in vitro translation, RT-PCR and cDNA synthesis
  • Cat.# Z5200 and Z5210 contain sufficient reagents for 3 separate mRNA isolations, each from 1-5mg total RNA
  • Cat.# Z5300 and Z5310 contain sufficient reagents for 15 separate mRNA isolations, each from 100-1,000µg total RNA

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Catalog number selected: Z5210

$ 283.00
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This product is discontinued
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This product is available under our Catalog (FT) program - Learn More
PolyATtract® mRNA Isolation Systems
3 isolations
$ 283.00
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The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The highly pure mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.

Specifications

You are viewing: Z5210 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119C 3 × 25ml View Product

SSC, 20X

Z525A 3 × 1.4ml

Biotinylated Oligo(dT) Probe

Z526A 1 × 35μl View Product

mRNA User Tube

Z528A 3 × 1 each

Streptavidin MagneSphere® Paramagnetic Particles

Z548F 3 × 3ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Specifications

You are viewing: Z5200 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119C 3 × 25ml View Product

SSC, 20X

Z525A 3 × 1.4ml

Biotinylated Oligo(dT) Probe

Z526A 1 × 35μl View Product

mRNA User Tube

Z528A 3 × 1 each

MagneSphere® Technology Magnetic Separation Stand (two-position)

Z5333 1 × 12 × 75mm

Streptavidin MagneSphere® Paramagnetic Particles

Z548F 3 × 3ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Specifications

You are viewing: Z5300 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119C 2 × 25ml View Product

SSC, 20X

Z525A 2 × 1.4ml

Biotinylated Oligo(dT) Probe

Z526B 1 × 50μl View Product

MagneSphere® Technology Magnetic Separation Stand (two-position)

Z5332 1 × 1.5ml

Streptavidin MagneSphere® Paramagnetic Particles

Z548C 15 × 0.6ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Specifications

You are viewing: Z5310 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119C 2 × 25ml View Product

SSC, 20X

Z525A 2 × 1.4ml

Biotinylated Oligo(dT) Probe

Z526B 1 × 50μl View Product

Streptavidin MagneSphere® Paramagnetic Particles

Z548C 15 × 0.6ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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