Notes: The authors investigated common variants of the APOA5 gene that have been associated with differences in plasma triglyceride (TG) levels. PCR fragments containing either the –1131T --> C promoter variant or containing both the –1131T --> C and –3G --> A promoter variants were cloned into the pGEM^®-T Vector System. The fragments were subsequently cloned into the pGL3 Basic Vector and transiently transfected into Huh7 and HepG2 cells along with the luciferase control vector, pRL-TK. The cells were lysed 48 hours after transfection and Luciferase activity was measured with the Dual-Luciferase^® Reporter Assay System. The function of the 1891T --> C variant in the 3´ UTR was tested the same way; with the exception that site-directed mutagenesis was performed to introduce the T --> C at position 1891 before the fragment was cloned into the pGL3 Basic Vector. The functionality of the Kozak sequence –3A --> G variant was determined by cloning cDNAs into the pGEM^®-7Zf Vector. Transcription/translation experiments were performed using the TNT^® Quick Coupled Transcription/Translation System and the proteins were labeled using the FluorTect™ Green_Lys System. In addition, a primer extension inhibition assay was performed using capped mRNAs generated with the Riboprobe^® System –T7 and the Ribo m⁷G Cap Analog. Ribosome binding reactions were performed using the Rabbit Reticulocyte Lysate System, Nuclease Treated. (3460)

Notes: The pGEM^®-7Zf(+) Vector was used for routine subcloning. (2244)

Notes: The pGEM^®-7Zf(+) Vector was used for routine subcloning. (0401)

Notes: The pGEM^®-7Zf(+) Vector was used for routine subcloning. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was used to asses viability following the growth of HPK1A and HPK1Aras cells in increasing amounts of the compound LG1069. (0351)

Notes: The pGEM^®-7Zf(+) Vector was used for routine subcloning. The resulting plasmid construct was used as a template for in vitro transcription. (0065)

Notes: Poly(A)+ RNA was purified with PolyATtract^® mRNA Isolation Systems. Anti-Rabbit IgG AP Conjugate, and BCIP/NBT were used in Western blotting. R transcripts were synthesized from the Lc cDNA in the pGEM-7Z(+) Vector, and were used in in vitro translation by Rabbit Reticulocyte Lysate Systems to be used as a positive control in Western. (0781)

Notes: Both the pGEM^®-5Zf(+) Vector and the pGEM^®-7Zf(+) Vector were used for routine subcloning of restriction fragments of the PEX20 gene in preparation for sequencing. (0269)

Notes: Poly A+ RNA was isolated from HeLa cell total RNA using the PolyATtract^® mRNA Isolation System and used for RT-PCR. (1699)