Soares, M.V.D., Borthwick, N.J., Maini, M.K., Janossy, G., Salmon, M., Akbar, A.N.
Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from CD45RA+ T cells isolated from cord blood. Cell samples (2-4 X 10^6 cells) were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at -70°C prior to isolation. The purified genomic DNA was used for Southern analysis. The DNA was also used for PCR amplification. (0346)