Wu, Q., Sandrock, T.M., Turgeon, B.G., Yoder, O.C., Wirsel, S.G., and Aist, J.R.
Notes: Genomic DNA was isolated from Nectria haematococca mycelia with the Wizard® Genomic DNA Purification Kit using a slightly modified protocol. Mycelia were ground in liquid nitrogen and lyophilized before lysing in a modified lysis buffer consisting of 50mM Tris/HCl, pH 7.5, 50 mM EDTA (pH 8.0), 3% SDS and 1% mercaptoethanol. The isolated DNA was the template for PCR. (0158)