Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard^® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin^® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard^® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

Notes: The authors used the Wizard^® Genomic DNA Purification Kit to isolate genomic DNA from rat FRTL-5 thyroid cells. (0282)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolated genomic DNA from P. denitrificans. The isolated DNA was used for Southern analysis. (0386)

Notes: Authors use the Wizard^® Genomic DNA Purification Kit to isolate DNA from newborn mice livers. They performed nested PCR on V(H)-D-J(H) genes and cloned these into the pGEM^®-T Vector and sequenced the rearrangements using the fmol^® DNA Cycle Sequencing System. (0908)

Notes: The Wizard^® Genomic DNA Purification Kit was used for isolation of chromosomal DNA from Vibrio cholerae. The isolated DNA was used for dot blot hybridization. (0359)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from S. pneumoniae with a slight modification to the standard protocol. The modification was the addition of 0.1% deoxycholate to the lysis solution followed by incubation for 10min. at 37°C. The isolated DNA was used for PCR. (0664)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate DNA from various Mycoplasma sp. The isolated DNA was used for Southern blotting. (1380)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-infected primary monkey fibroblasts. The isolated DNA was used for PCR. The standard protocol was modified somewhat in that the cells were lysed in 300µl of Nuclei Lysis Solution containing proteinase K overnight at 37°C. The DNA was resuspended at the equivalent of 500, 1000 and 2000 cells per microliter. (0729)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 10⁹ cells/ml. The isolated DNA was used for AFLP analysis. (1203)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from E. coli strain JRG3983. The isolated DNA was analyzed by Southern blotting. (1109)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from the 45-16 leukemic cell line. The isolated DNA was used for Southern analysis. (1450)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-immortalized T cells. The isolated DNA was used for PCR. (0483)

Notes: Genomic DNA was isolated from mouse brain with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin^® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate viral DNA and genomic DNA from Vero cells infected with HSV-1. The isolated DNA was used for PCR. (1393)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from control and apoptotic RAW 264.7 cells. The apoptotic cells had a laddered appearance consistent with apoptosis. DNA isolated from control cells was of high molecular weight with no appearance of laddering. (0951)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Vibrio harveyi. The isolated DNA was used for PCR. (0060)

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard^® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

Notes: Genomic DNA was isolated from Nectria haematococca mycelia with the Wizard^® Genomic DNA Purification Kit using a slightly modified protocol.  Mycelia were ground in liquid nitrogen and lyophilized before lysing in a modified lysis buffer consisting of 50mM Tris/HCl, pH 7.5, 50 mM EDTA (pH 8.0), 3% SDS and 1% mercaptoethanol.  The isolated DNA was the template for PCR. (0158)

Notes: Chromosomal DNA was obtained from E. coli using the Wizard^® Genomic DNA Purification Kit. The DNA was used for EMBL4 library construction as well as Southern analysis. (1490)

Notes: The Wizard^® Lambda DNA Purification System was used to isolate phage for the Sphingomonas paucimobilis and Flavobacterium sp. isolates. The isolated DNA was used for determination of the phage genome size by restriction digestion. The Wizard^® Genomic DNA Purification Kit was used to isolate the genomic DNA from these bacterial species as well. The authors note that the DNA isolated with the Wizard^® Genomic Kit was as pure as that purified by a cesium chloride gradient. Loading of the DNA isolated with the Genomic Kit onto cesium chloride gradients produced only a single sharp band after centrifugation. The isolated DNA was used for dot blot hybridizations. (0979)

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from Haemophilus influenzae. The isolated DNA was used for amplification. (1310)

Notes: Genomic DNA was isolated from Methanococcus jannaschii using the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR and eventually expression of the FtsZ protein. (0746)

Notes: Human genomic DNA was isolated from blood with the Wizard^® Genomic DNA Purification System for linkage analysis of 300 polymorphic markers. (0399)