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Citations Search

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Infect. Immun. 68, 7180-7185. Phylogeny of Vibrio cholerae based on recA sequences. 2000

Stine, O.C., Sozhamannan, S., Gou, Q., Zheng, S., Morris, J.G., Jr., and Johnson, J.A.

Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

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J. Clin. Microbiol. 38(8), 2829-2836. Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon 2000

Park, S., Wong, M., Marras, S. A. E., Cross, E.W., Kiehn, T.E., Chaturvedi, V., Tyagi, S., and Perlin, D.S.

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

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Genetics 155, 1149-1160. RNA editing of the Drosophila para Na+ channel transcript: Evolutionary conservation and developmental regulation. 2000

Hanrahan, C.J., Palladino, M.J., Ganetsky, B. and Reenan, R.A.

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

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Proc. Natl. Acad. Sci. USA 96, 2285-2290. Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides. 1999

Suzuki, K., Mori, A., Ishii, K.J., Saito, J., Singer, D.S., Klinman, D.M., Krause, P.R. and Kohn, L.D.

Notes: The authors used the Wizard® Genomic DNA Purification Kit to isolate genomic DNA from rat FRTL-5 thyroid cells. (0282)

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J. Bacteriol. 181, 6907-6913. Anaerobic growth of Paracoccus denitrificans requires cobalamin: Characterization of cobK and cobJ genes. 1999

Shearer, N., Hinsley, A.P., Van Spanning, R.J.M., Spiro, S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from P. denitrificans. The isolated DNA was used for Southern analysis. (0386)

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J. Immunol. 162, 1566-1572. Atypical VH-D-JH rearrangements in newborn autoimmune MRL mice. 1999

Klonowski, K.D., Primiano, L.L., Monestier, M.

Notes: Authors use the Wizard® Genomic DNA Purification Kit to isolate DNA from newborn mice livers. They performed nested PCR on V(H)-D-J(H) genes and cloned these into the pGEM®-T Vector and sequenced the rearrangements using the fmol® DNA Cycle Sequencing System. (0908)

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Infect. Immun. 67, 5033-5040. Cloning and sequencing of the genes downstream of the wbf gene cluster of Vibrio cholerae serogroup O139 and analysis of the junction genes in other serogroups. 1999

Sozhamannan, S., Deng, Y.K., Li, M., Sulakvelidze, A., Kaper, J.B., Johnson, J.A., Nair, G.B., Morris, J.G.Jr.

Notes: The Wizard® Genomic DNA Purification Kit was used for isolation of chromosomal DNA from Vibrio cholerae. The isolated DNA was used for dot blot hybridization. (0359)

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J. Bacteriol. 181, 5355-5364. Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae type belonging to serogroup 19. 1999

Morona, J.K., Morona, R., Paton, J.C.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from S. pneumoniae with a slight modification to the standard protocol. The modification was the addition of 0.1% deoxycholate to the lysis solution followed by incubation for 10min. at 37°C. The isolated DNA was used for PCR. (0664)

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Infect. Immun. 67, 760-771. Differential posttranslational processing confers intraspecies variation of a major surface lipoprotein and a macrophage-activating lipopeptide of Mycoplasma fermentans 1999

Calcutt, M.J., Kim, M.F., Karpas, A.B., Muhlradt, P.F., Wise, K.S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate DNA from various Mycoplasma sp. The isolated DNA was used for Southern blotting. (1380)

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J. Virol. 73, 10320-10328. Experimental infection of Rhesus and pig-tailed macaques with macaque rhadinoviruses 1999

Mansfield, K.G., Westmoreland, S.V., DeBakker, C.D., Czajak, S., Lackner, A.A., Desrosiers, R.C.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate total DNA from virus-infected primary monkey fibroblasts. The isolated DNA was used for PCR. The standard protocol was modified somewhat in that the cells were lysed in 300µl of Nuclei Lysis Solution containing proteinase K overnight at 37°C. The DNA was resuspended at the equivalent of 500, 1000 and 2000 cells per microliter. (0729)

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Appl. Environ. Microbiol. 65, 2369-2375. High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. 1999

Duim, B., Wassenaar, T.M., Rigter, A., Wagenaar, J.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 109 cells/ml. The isolated DNA was used for AFLP analysis. (1203)

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J. Bacteriol. 181, 1238-1248. Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-decarboxylates in Escherichia coli. 1999

Golby, P., Davies, S., Kelly, D.J., Guest, J.R., Andrews, S.C.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from E. coli strain JRG3983. The isolated DNA was analyzed by Southern blotting. (1109)

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J. Virol. 73, 2038-2044. Identification of protein instability determinants in the carboxy-terminal region of c-myb removed as a result of retroviral integration in murine monocytic leukemias. 1999

Bies, J., Nazarov, V. and Wolff, L.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate total DNA from the 45-16 leukemic cell line. The isolated DNA was used for Southern analysis. (1450)

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J. Virol. 73, 4856-4865. Immortalization of CD4+ and CD8+ T lymphocytes by human T-cell leukemia virus type 1 tax mutants expressed in a functional molecular clone. 1999

Robek, M.D., Ratner, L.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate total DNA from virus-immortalized T cells. The isolated DNA was used for PCR. (0483)

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J. Clin. Invest. 104, 647-656. Inducible nitric oxide synthase is an endogenous neuroprotectant after traumatic brain injury in rats and mice. 1999

Sinz, E.H., Kochanek, P.M., Dixon, C.E., Clark, R.S.B., Carcillo, J.A., Schiding, J.K., Chen, M., Wisniewski, S.R., Carlos, T.M., Williams, D., DeKosky, S.T., Watkins, S.C., Marion, D.W., Billiar, T.R.

Notes: Genomic DNA was isolated from mouse brain with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

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J. Virol. 73, 3843-3853. Intrastrain variants of herpes simplex virus type 1 isolated from a neonate with fatal disseminated infection differ in the ICP34.5 gene, glycoprotein processing and neuroinvasiveness. 1999

Bower, J.R., Mao, H., Durishin, C., Rozenbom, E., Detwiler, M., Rempinski, D., Karban, T.L., Rosenthal, K.S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate viral DNA and genomic DNA from Vero cells infected with HSV-1. The isolated DNA was used for PCR. (1393)

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J. Immunol. 162, 3395-3401. Overexpression of protein kinase C isoforms protects RAW 264.7 macrophages from nitric oxide-induced apoptosis: Involvement of c-jun N-terminal kinase/stress-activated protein kinase, p38 kinase, and CPP-32 protease pathways. 1999

Jun, C.-D., Oh, C.-D., Kwak, H.-J., Pae, H.-O., Yoo, J.-C., Choi, B.-M., Chun, J.-S., Park, R.-K., Chung, H.-T.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from control and apoptotic RAW 264.7 cells. The apoptotic cells had a laddered appearance consistent with apoptosis. DNA isolated from control cells was of high molecular weight with no appearance of laddering. (0951)

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Biochemistry 38, 16246-16252. Sequencing and preliminary characterization of the Na+-translocating NADH: Ubiquinone oxidoreductase from Vibrio harveyi 1999

Zhou, W., Bertsova, Y.V., Feng, B., Tsatsos, P., Verkhovskaya, M.L., Gennis, R.B., Bogachev, A.V., Barquera, B.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Vibrio harveyi. The isolated DNA was used for PCR. (0060)

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Am. J. Hum. Genet. 65, 1547–1560. The molecular basis of Sjogren-Larsson syndrome: Mutation analysis of the fatty aldehyde dehydrogenase gene. 1999

Rizzo, W. B., Carney, G. and Lin, Z.

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

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Mol. Biol. Cell 9, 89-101. A fungal kinesin required for organelle motility, hyphal growth, and morphogenesis. 1998

Wu, Q., Sandrock, T.M., Turgeon, B.G., Yoder, O.C., Wirsel, S.G., and Aist, J.R.

Notes: Genomic DNA was isolated from Nectria haematococca mycelia with the Wizard® Genomic DNA Purification Kit using a slightly modified protocol.  Mycelia were ground in liquid nitrogen and lyophilized before lysing in a modified lysis buffer consisting of 50mM Tris/HCl, pH 7.5, 50 mM EDTA (pH 8.0), 3% SDS and 1% mercaptoethanol.  The isolated DNA was the template for PCR. (0158)

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J. Biol. Chem. 273, 8376-8381. A rare 920-kilobase chromosomal inversion mediated by IS1 transposition causes constitutive expression of the yiaK-S operon for carbohydrate utilization in Eschericia coli. 1998

Badía, J., Ibáñez, E., Sabaté, M., Baldomà, L. and Aguilar, J.

Notes: Chromosomal DNA was obtained from E. coli using the Wizard® Genomic DNA Purification Kit. The DNA was used for EMBL4 library construction as well as Southern analysis. (1490)

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Appl. Environ. Microbiol. 64, 535-542. Characterization of marine temperate phage-host system isolated from Mamala Bay, Oahu, Hawaii. 1998

Jiang, S.C., Kellogg, C.A., Paul, J.H.

Notes: The Wizard® Lambda DNA Purification System was used to isolate phage for the Sphingomonas paucimobilis and Flavobacterium sp. isolates. The isolated DNA was used for determination of the phage genome size by restriction digestion. The Wizard® Genomic DNA Purification Kit was used to isolate the genomic DNA from these bacterial species as well. The authors note that the DNA isolated with the Wizard® Genomic Kit was as pure as that purified by a cesium chloride gradient. Loading of the DNA isolated with the Genomic Kit onto cesium chloride gradients produced only a single sharp band after centrifugation. The isolated DNA was used for dot blot hybridizations. (0979)

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Infect. Immun. 66, 656-663. Comparative analysis of Haemophilus influenzae hifA (pilin) genes 1998

Clemans, D.L., Marrs, C.F., Patel, M., Duncan, M., Gilsdorf, J.R.

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from Haemophilus influenzae. The isolated DNA was used for amplification. (1310)

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Nature 391, 203-206. Crystal structure of the bacterial cell-division protein FtsZ. 1998

Löwe, J., Amos, L.A.

Notes: Genomic DNA was isolated from Methanococcus jannaschii using the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for PCR and eventually expression of the FtsZ protein. (0746)

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Am. J. Hum. Genet. 63, 101-108. Evidence for linkage of human primary systemic carnitine deficiency with D5S436: a novel gene locus on chromosome 5q. 1998

Shoji, Y., Koizumi, A., Kayo, T., Ohata, T., Takahashi, T., Harada, K., Takada, G.

Notes: Human genomic DNA was isolated from blood with the Wizard® Genomic DNA Purification System for linkage analysis of 300 polymorphic markers. (0399)

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