Notes: The Wizard^® Genomic DNA Purification Kit was used to purify Bacillus anthracis chromosomal DNA, which was then used in PCR to amplify a luxN ortholog (named open reading frame BA5047). Amplified DNA containing the luxN ortholog region was then cloned into the pGEM^®-T Easy Vector. This construct, when transformed into Vibrio harveyi (AI-1^-, and AI-2⁺), allowed the detection of an upregulated signaling system by a bioluminescent assay. (3092)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from various Streptococcal strains (Streptococcus sanguinis, S. oralis, S. gordonii, S. cristatus, S. mitis, S. parasanguinis, S. pneumoniae, S. mutans, S. salivarius, S. sobrinus, S. ratti, and S. vestibularis), Lactobacillus acidophilus, Actinomyces naeslundii, and E. coli JM109. The genomic DNA was diluted to 50μg/ml and used in Arbitrarily Primed PCR (AP-PCR) experiments to identify common amplimers from clinical Streptococcus sanguinis samples. The authors were able to identify a specific probe that may be helpful in identification of  S. sanguinis clinical isolates. (2836)

Notes: The Wizard^® Genomic DNA Purification System was used to isolate genomic DNA from clinically isolated Haemophilus influenzae samples.  The genomic DNA was then digested with EcoRI and used in Southern blots to identify the presence of various Haemophilus influenzae adhesin genes. (3102)

Notes: Genomic DNA was isolated from Madurella mycetomatis samples.  A modified  Wizard^® Genomic DNA Purification Kit protocol was used for the isolations.  Mycelia were sonicated and ground in liquid nitrogen before the addition of Nuclei Lysis Solution. The purified DNA was used in PCR.  (3049)

Notes: Cow (Bos taurus), horse (Equus caballus), sheep (Ovis aries), antelope (Antilocapra americana), dog (Canis familiaris), cat (Felis catus), and rabbit (Oryctolagus cuniculus) genomic DNA were obtained by extraction from tissue and blood samples using the Wizard^® Genomic DNA Purification kit. SINEs (short interspersed elements) PCR was performed on various combinations of purified DNA from the above species. (3260)

Notes: The authors of this paper describe using the Wizard^® Genomic DNA Purification Kit to isolate genomic DNA from Helicobacter pylori. The isolated genomic DNA was used in PCR to analyze motB gene mutations.  (3101)

Notes: Genomic DNA was isolated from H. pylori with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used in both PCR amplification and Southern blotting. (2292)

Notes: In this study, the Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Bacillus subtilis. (2428)

Notes: The authors describe using the Wizard^® Genomic DNA Purification kit and the SV Total RNA Isolation System to isolate genomic DNA and total RNA, respectively, from Streptococcus pneumoniae. The researchers also added RNasin^® Ribonuclease Inhibitor to purified total RNA before storing it and using it for later RT-PCR reactions performed with the Access RT-PCR System. (2835)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from individual Tribolium cataneum flour beetles. The isolated genomic DNA was used for PCR amplification for recombinational mapping related to eye color, PCR-based deletion breakpoint analysis, and was EcoR I-digested for Southern blotting analysis. (2504)

Notes: The authors report an enrichment of microorganisms belonging to the Geobacteraceae family on energy-harvesting anodes from marine sediments. Electrodes were rinsed under sterile marine buffer then scraped with sterile razor blades to remove microorganisms. DNA was extracted using an organic extraction method and then further purified using a Wizard^® Genomic DNA Purification Kit. (2592)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify DNA from two strains of Aspergillus fumigatus.  A modified protocol was used for the purification.  Cells were filtered through a 0.2μm SFCA filter, frozen on dry ice and then ground with a mortar and pestle.  The ground cells were then incubated  in Nuclei Lysis Solution at 37°C for 1 h before continuing with the Wizard^® Genomic DNA Tissue Culture Cell procedure.  The purified DNA was used in PCR with primers for the A. fumigatus PMA1 gene. (3048)

Notes: Genomic DNA was isolated from Leptospira kirschneri with the Wizard® Genomic DNA Purification Kit.  The isolated DNA was used for Southern blotting. (2296)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from wildtype and engineered mutants of B. bronchiseptica. The isolated genomic DNA was used in PCR to analyze the size of the dnt gene. The engineered deletion mutants produced a 1.7kb PCR product and the wildtype gene produced a 2.3kb product. (2293)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from 5ml exponentially growing E. coli cultures.  The isolated DNA was used for Southern blotting. (2298)

Notes: Genomic DNA was isolated from Serratia marcescens and Mycobacterium parafortuitum with the Wizard® Genomic DNA Purification Kit.  Isolation from Serratia was straightforward following the recommended protocol in Technical Manual TM050.  Mycobacterium cells were first treated with proteinase K and lysozyme followed by addition of glass beads. After vigorous shaking in a bead beater, genomic DNA was isolated using the standard protocol from then on. The isolated DNA was quantitated by a DAPI-based quantitation for irradiated and nonirradiated DNA. (2290)

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from Salmonella enterica serovars.  The isolated DNA was used as template in PCR reactions with 49 primer sets to examine the structure of the rrn operon. Some amplimers were up to 7kb in size. (2297)

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from 5ml early stationary phase cultures of Borrelia burgdorferi.  Isolated genomic DNA was used in both Southern blotting and PCR. (2294)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate target genomic DNA from C. jejuni human isolates for microarray analysis.  The isolated DNA was used as a template for Cy®3- and Cy®5-dCTP incorporation by random primed labeling reactions with Klenow. (2295)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolated genomic DNA from 5 x 10⁷ human G361 melanoma cells. The isolated DNA was used for Southern blotting. Probes were constructed with the DNA 5'-End Labeling System. The cDNA for the H11 protein was expressed in 293 HEK cells using the pCI Mammalian Expression Vector. (0075)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolated genomic DNA from D. desulfuricans. The isolated DNA was used for Southern analysis and cosmid library construction. (0499)

Notes: In this paper, the Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from various tissues including rat skeletal muscle, liver and heart. Purification was performed with 30-50mg of tissue. The isolated tissue was used for Southern analysis of mitochondrial DNA sequences. (2233)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Mycoplasma hyorhinis. The isolated DNA was used for PCR. (1306)

Notes: The Wizard^® Genomic DNA Purification Kit was used to purify genomic DNA from the archaeabacterium, Methanosarcina mazei S-6.  The isolated genomic DNA was used in PCR to create templates for sequencing reactions.  (3106)