Notes: Total RNA was isolated from various rat tissues with the SV Total RNA Isolation System. Yields are reported as 10µg from 16 whole cochlea, 8µg from 16 modiola, 10.4µg from 48 cochlear sensorineural epithelial/lateral walls and 50µg from the substantia nigra region of four brains. The isolated RNA was used for RT-PCR in the presence of RNasin^® Ribonuclease Inhibitor. The resulting amplimer was subcloned into the pGEM^®-T Easy Vector and clones were purified with the Wizard^® Plus SV Minipreps System. (2176)

Notes: Total RNA was isolated from 10⁶ actively growing Jurkat, MAW, OZZ or Thp1 cells with the SV Total RNA Isolation System. The equivalent RNA from 8 x 10⁴ cells was used for RT-PCR with the Access RT-PCR System. (2180)

Notes: The SV RNA Total RNA Isolation System was used to isolate total RNA from rat PC12 cells. The isolated RNA was used for RT-PCR in the Access RT-PCR System. (0083)

Notes: The SV Total RNA Isolation System was used to purify total RNA from 30–60mg of human adenoma tumor tissue. The isolated RNA was used for RT-PCR and a Taqman assay. (2177)

Notes: Total RNA was isolated from Rhizobium leguminosarum bv. trifolii using the SV Total RNA Isolation System. This RNA was used in a primer extension assay using AMV Reverse Transcriptase to determine the transcriptional start site of the mat operon. Nested deletions during the sequencing of the mat promoter were prepared. The firefly luciferase gene from pGL3 Basic was used to construct a reporter construct containing the mat promoter to study transcriptional effects of MatR. The interaction of the MatR protein with the mat operator was mapped. (2308)

Notes: Total RNA was isolated from Neiserria meningitidis using the SV Total RNA Isolation System. The relative levels of crgA transcript in crgA mutants and wild type strains was determined by RT-PCR with the Access RT-PCR System. (2309)

Notes: Total RNA was isolated from the CNS, spleen and small intestine of mice with preclinical, acute, remission and relapse EAE with the SV Total RNA Isolation System. The isolated RNA was used to analyze gene expression in each stage using the Reverse Transcriptase System and Taq DNA Polymerase in two step RT-PCR. (2163)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from 100mg of pea seedling leaves. The isolated RNA was used for Northern blots and RT-PCR. Full transcripts of the Pea enation mosaic virus-1 and Pea enation mosiac virus-2 were produced with T7 RNA Polymerase in vitro and translated in Rabbit Reticulocyte Lysate. (2174)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from freshly isolated human CD4+ T cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. (2183)

Notes: The SV Total RNA Isolation System was used to isolate RNA from transfected NIH3T3 cells. The isolated RNA was used for RT-PCR. (2159)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from rat kidney medulla and medullary thick ascending limb. The isolated RNA was used in a quantitative RT-PCR. (0073)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from 3T3-F442A preadipocytes after various treatments. The isolated RNA was used for Northern analysis. (2181)

Notes: The SV Total RNA Isolation System was used to isolate RNA from various lung cancer cell lines. The isolated RNA was used for RT-PCR and quantitative RT-PCR. (2161)

Notes: Total RNA was isolated from human serum by combining 100µl of serum with 175µl of SV RNA Lysis Buffer. Only fresh or once-frozen serum was used. The SV Total RNA Isolation System was also used to isolate total RNA from tumor tissue. The rest of the protocol was followed as directed in the technical manual. The quantities of RNA isolated from serum were too low to quantitate so 1µl or 5µl of the isolated RNA was used in RT-PCR to analyze RNA content. (2160)

Notes: Total RNA was isolated from a virulent strain of Salmonella enterica, SL1344 using the SV Total RNA Isolation System. The Access RT-PCR System was used to characterize the transcriptional organization of the prg operon. The pgrH gene was amplified by RT-PCR and cloned into the pGEM^®-T vector (2305)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from various rat tissues. The isolated RNA was used for RT-PCR. The authors also use a derivative of the pCI Vector called pCI-IRES-CD8 to express the TWIK protein specifically in COS cells. (2179)

Notes: The SV Total RNA isolation System was used to isolate total RNA from HeLa human cervical carcinoma cells, COS-7 SV40-transformed monkey kidney cells, MDBK bovine kidney cells and MDCK canine kidney cells. The isolated RNA was used for Northern blot analysis. (0543)

Notes: Total RNA was isolated from uninfected Vero cells and Vero cells infected with either Simkania negevensis Z^T or Chlamydia trachmatis using the SV Total RNA Isolation System. The isolated RNA was used in RT-PCR amplifications to determine the size of the unspliced intron form of the 23S rRNA to differentiate between a group I intron where the rRNA intron is spliced with religation and an intervening segment where the rRNA is fragmented to functionally remove the intervening segments. The isolated RNAs and Promega's RNA Markers were separated by electrophoresis to judge RNA integrity. (2302)

Notes: The SV Total RNA Isolation System was used to isolate RNA from human peritoneal mesothelial cells. The isolated RNA was used for RT-PCR. (2164)

Notes: Taq DNA Polymerase was used extensively to clone the BLINaC cDNA. The resulting clone was put into the pCI Mammalian Expression Vector. Total RNA was isolated from rat liver and primary hepatocytes with the SV Total RNA Isolation System. The RNA was used for RT-PCR. Northern blotting was performed with poly-A(+) RNA isolated with the PolyATtract^® mRNA Isolation System. (0472)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human cerebrovascular tissue removed during surgery. The isolated RNA was used for first strand cDNA synthesis for the SAGE procedure. (2178)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from gingivostomatitis tissue. The RNA was eluted from the basket with 35µl of water, which was passed through the basket three times. Eleven microliters of the eluted material was used for two-step RT-PCR. One microliter of each eluted sample was analyzed for genomic DNA contamination by PCR, and any samples that did amplify were put through the SV RNA System again. As reported by the authors, 'Genomic DNA contamination was undetectable in the vast majority of sample eluates after a single RNA extraction procedure. The remaining samples were subsequently shown to be free from detectable genomic DNA after RNA reextraction.' (1064)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from various mouse cells lines: DA3 IL-3 dependent myeloid cells; 7TD1 IL-6-dependent B-cell hybridoma; FM3A mammary carcinoma; L5287Y lymphoma; WEHI-3 myeloma; LLC1 Lewis lung carcinoma; L929 fibroblast; J774A macrophage-like cells. Five micrograms of each RNA was used for Northern analysis. The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to determine if HL60 were apoptotic when treated with retionic acid to induce growth arrest. Less than 10% of the cells were positive in the TUNEL assay (data not shown). (0113)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

Notes: Total RNA was isolated from Lactobacillus helveticus for use in 5´ RACE (Rapid Amplification of cDNA Ends) to map the 5' end of the prtH RNA which encodes a cell envelope associated proteinase involved in casein hydrolysis. (2303)